FastQCFastQC Report
Wed 25 May 2016
SRR1779903_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779903_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424617
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG245315.777206282367404No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG69781.6433633132917431No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG9400.22137596940301493No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC8960.2110136899841504Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT7890.18581451048827533No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGTA200.00694801552.50194554
TGGCGTA200.00694801552.50194524
CTTAGCG200.00694801552.50194530
TAACGGT200.00695125252.4957648
CGACAGT200.00695125252.4957647
GCCGTAT11650.046.8686954
CGTTTTA305.8775325E-446.662916
TGGTCGC11900.046.4723149
TCGCCGT11750.046.46980752
CCGTATC11800.046.272955
CGCCGTA11850.046.07765653
CTCGGTG11900.045.87864744
GTCGCCG12050.045.60334851
TCGGTGG12050.045.5979845
CGTATCA12000.045.50168256
TCTCGGT12000.045.49632643
GTGGTCG12200.045.32420748
CGGTGGT12250.045.1392146
GTAGATC12450.044.4193138
GGTCGCC12500.043.96162850