FastQCFastQC Report
Wed 25 May 2016
SRR1779903_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779903_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences424617
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT119852.8225436098884407No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115202.7130331569390767No Hit
ATTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT36000.8478228615434615No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG19740.4648895357463314No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC14160.33347699220709487No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT4570.10762640214593386TruSeq Adapter, Index 2 (95% over 21bp)

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTACCCG150.002225048869.9911659
AGGGGGG19550.060.6828870
TACCCGC308.377294E-658.3259710
CGTCTTA1200.055.4096748
TGGTTCG200.006952502352.49337458
CCGTATC200.006952502352.49337414
GTCCCGT200.006952502352.49337411
GCCGTCT22300.050.5317446
CCGTCTT22250.050.4880147
TATGCCG24550.049.8918743
CGTCTTC21750.049.71786548
ATGCCGT25200.049.29933544
CGTATGC24350.049.29562841
TGCCGTC24700.049.16383745
TCGTATG25000.049.13379740
CTCGTAT25150.049.11905339
TCTCGTA25800.048.1528438
GTATGCC25450.048.1275242
ATCTCGT25900.048.1020437
GTCTTCT21400.047.58745649
Produced by FastQC (version 0.11.5)