FastQCFastQC Report
Wed 25 May 2016
SRR1779902_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779902_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347590
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG228276.567220000575391No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG53541.5403204925343077No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC9200.26467965131332893Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG6710.19304352829483012No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT5980.1720417733536638No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTTTA200.006947976552.50000431
ACTCTCG200.006947976552.5000049
GCATTCG200.006947976552.5000041
GCCGTAT8050.046.08695654
TCGGTGG8350.045.26946345
TGGTCGC8350.045.26946349
TCGCCGT8200.045.24390452
CGGTGGT8450.045.14792646
CGCCGTA8350.044.43113753
CGTATCA8300.044.2771156
GCGAACT404.5881592E-543.75000428
CTCGGTG8750.043.644
ATCTCGG8600.043.54651342
CCGTATC8450.043.4911255
TCTCGGT8700.043.44827343
GTCGCCG8650.043.29479651
GTATCAT8250.043.27272457
GATCTCG8750.042.841
GTAGATC8800.042.5568238
TAGATCT8900.042.0786539