FastQCFastQC Report
Wed 25 May 2016
SRR1779902_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779902_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences347590
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT104723.012744900601283No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG94502.718720331424955No Hit
ATTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT46151.3277136856641445RNA PCR Primer, Index 19 (95% over 21bp)
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG17590.5060559855001583No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC12440.35789291981932736No Hit
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT5760.16571247734399722No Hit
ATCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC3940.11335193762766478No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCGGAT150.002225208669.98723
AGGGGGG20150.058.70738670
ACGCGTA200.00692535252.54331227
CGCGTAA200.00693324552.5281828
GTTTCGC200.006953005752.49041
TAGCGCG200.006953005752.49042
CGGATTG200.006953005752.49045
CAGTATA200.006953005752.49041
TATGCCG22800.051.2625543
GCCGTCT22500.051.0128946
CGTCTTC22700.050.71759448
CCGTCTT22500.050.70183647
GTATGCC23100.050.59680642
ATGCCGT23100.050.59680644
CGTATGC23350.050.35481641
TCGTATG23850.050.33346640
TGCCGTC23200.050.2278845
CTCGTAT23850.050.03997439
TCTCGTA24000.049.72722238
ATCTCGT24100.049.37566837