FastQCFastQC Report
Wed 25 May 2016
SRR1779901_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779901_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392299
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG339638.65742711554197No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG82242.0963601742548414No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT10020.2554174239546876No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC9870.25159380982362944Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG8900.22686777177611975No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGTAC200.006945837852.50535615
CCGCGAT200.006945837852.50535628
AGCGTCG200.006949340552.4986625
TAGCGAC200.006949340552.4986655
TGACGAA200.006949340552.4986612
TGGTCGC14000.047.74878349
GCCGTAT13650.047.69109354
CGGTGGT14050.047.08065446
CGTATCA13900.046.83333656
TCTCGGT13900.046.83333643
GTGGTCG14200.046.82979248
CGCCGTA13950.046.66547453
TCGCCGT13950.046.41458552
GTCGCCG14050.046.33334451
CTCGGTG14150.046.2532444
CCGTATC14050.046.0842455
TCGGTGG14350.045.85249345
GGTGGTC14650.045.39133547
GATCTCG14300.045.2785641
GTAGATC14700.045.23694238
Produced by FastQC (version 0.11.5)