FastQCFastQC Report
Wed 25 May 2016
SRR1779901_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779901_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392299
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT169984.33291953331515No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG139593.558255310362759No Hit
ATTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT52571.3400492991315298No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG29840.7606443044718442No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC13390.3413212880991285No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT8810.22457360329748485No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC5410.13790501632683236No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT4630.11802222284532972No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4030.10272776632109692No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATACGCG150.002224338769.995812
CGCTTCG150.002224338769.995845
AGGGGGG28700.061.10167770
GTACCGA200.006950292752.4968457
AGTAGCG200.006950292752.4968452
AAGGGGG33800.051.6750869
TATGCCG35250.050.43668443
ATGCCGT35400.050.42069644
CGTATGC35600.050.23574441
TGCCGTC35550.050.20795445
CGTCTTC35400.050.13049348
CCGTCTT35100.050.05397447
TCGTATG36150.050.0523240
GTATGCC35700.049.99699442
CTCGTAT35800.049.85733839
TCTCGTA35600.049.744238
GCCGTCT35600.049.744246
ATCTCGT35500.049.68715337
AATCTCG35850.049.59255636
ATCGTAT506.190385E-848.9970639