FastQCFastQC Report
Wed 25 May 2016
SRR1779900_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779900_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212479
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2868013.497804488914197No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG66913.149017079334899No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC11370.5351117051567449Illumina Single End PCR Primer 1 (95% over 21bp)
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT7150.33650384273269357No Hit
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG6980.32850305206632185No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTGGA150.002222402370.07
TGCGGAC150.002222402370.011
GCTCTTG150.002222402370.058
AGGTCCA150.002222402370.011
CGGACCG150.002222402370.013
AACCTGG252.3865879E-455.99999650
CGATAGA252.3865879E-455.99999637
CTGCGGA200.006944278752.510
TTGAAGG200.006944278752.552
GAGTTAG407.455237E-752.520
GCGGACC200.006944278752.512
GATTCAA200.006944278752.526
AGTTAGC451.6792619E-646.66666821
TACATCT305.868529E-446.66666458
TCTCCAG305.868529E-446.66666418
CGCAATA305.868529E-446.66666457
TCGCCGT11100.046.35135352
TCTCGGT11800.046.27118743
GTCGCCG11250.046.04444551
CTCGGTG11450.045.8515344
Produced by FastQC (version 0.11.5)