FastQCFastQC Report
Wed 25 May 2016
SRR1779900_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779900_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences212479
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT142486.705603847909676No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG115315.42688924552544No Hit
ATTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT45512.1418587248622214No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG23491.1055210161945417No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC15490.7290132201299893No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT6610.3110895664983363TruSeq Adapter, Index 7 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC5340.2513189538730886No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3870.18213564634622714No Hit
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT3570.16801660399380644No Hit
CTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTC2440.11483487779968843No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCCGCA150.002220831370.01247426
GACTCCG150.00222290669.9959957
ATATCGT453.8380676E-1062.21866237
ATCGTAT401.0621079E-861.246539
GCCGTAT308.359533E-658.32999446
AGGGGGG24450.057.97214570
TCCGGTC252.3872616E-455.9968038
ATGGGTC200.006939383752.50935730
GGTCCGC200.006939383752.50935725
AGCGATC200.006939383752.50935731
CGAGAAC200.006939383752.50935723
CGGTCCG200.006939383752.50935724
TGTGGCA200.00694584852.49699838
CGTCTAC200.00694584852.49699848
TGTGCAC200.00694584852.49699849
CTGGTTA200.00694584852.49699861
CGTTATC200.00694584852.49699810
TCGTCAC200.00694584852.49699815
GCGGCCA200.00694584852.49699810
GACGCTA200.00694584852.49699810
Produced by FastQC (version 0.11.5)