FastQCFastQC Report
Wed 25 May 2016
SRR1779899_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779899_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408184
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG312767.662230758677459No Hit
CTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTG76741.8800344942476923No Hit
ATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGTGGTC12290.30108970464300416Illumina Single End PCR Primer 1 (95% over 21bp)
CTCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGG8720.2136291476392999No Hit
TCTTATACACATCTGACGCTGCCGACGAAGAGGATAGTGTAGATCTCGGT8050.19721498147894084No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TGTGCAA252.39152E-455.98902510
GGGCCGA200.006944127452.50900732
TGGCACT200.006947493652.50257541
TTTATAA200.006950861352.4961430
TCGCCGT13500.044.59477652
GCCGTAT13500.044.58385554
GTCGCCG13650.044.3611551
TGGTCGC13650.043.848349
ATCTCGG13650.043.5918842
CTCGGTG13650.043.5918844
CGGTGGT13750.043.52940846
TCGGTGG13750.043.52940845
GATCTCG13700.043.43278541
AGATCTC13750.043.2748540
CGTATCA13850.043.21511556
GTAGATC13850.043.21511538
CGCCGTA14100.042.9453753
TCTCGGT13900.042.80785443
GTATCAT14000.042.2520757
GATCCTA757.566996E-1041.9917681