FastQCFastQC Report
Wed 25 May 2016
SRR1779899_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779899_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences408184
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT157603.861003861003861No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG132643.2495149246418285No Hit
ATTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT46931.1497265938890304No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG28060.6874350782980224No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC16640.4076592909080219No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT9250.22661348803480783RNA PCR Primer, Index 19 (95% over 23bp)
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC5210.12763851596338907No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT5060.12396370264390569No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGAA150.002216532470.0583728
CGTCTAC252.8422564E-669.9811348
AGGGGGG28700.057.6673870
CTTTATA1000.055.9917682
CGTTTCA200.006946146552.50514658
ATCGTAT407.486269E-752.4858539
CGTCTTC32050.051.85809348
GCCGTCT31900.051.7728846
CCGTCTT31600.051.7107447
TATGCCG32550.051.38400343
CGTATGC32900.051.15641841
TCGTATG33450.051.1521340
TCTTCTG32600.050.8758650
CTTGAAA32900.050.8560557
CTCGTAT33350.050.7809139
GTATGCC33050.050.6066342
TCTCGTA33600.050.50721438
TGCCGTC32850.050.4886745
ATGCCGT33100.050.31876444
CTGCTTG32850.050.2879654