FastQCFastQC Report
Wed 25 May 2016
SRR1779898_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779898_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329121
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG314179.545729382202898No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG26270.7981866851401157No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC4580.13915854655278756No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3580.10877458442335797No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGATCC200.006948069752.4992034
GCCGTAT4850.045.4632354
TGGTCGC4850.045.4632349
TCGGTGG4800.045.20764545
GATCTCG4750.044.94668641
TCGCCGT4850.044.7415952
AGATCTC4800.044.4784940
CGGTGGT5150.043.49448446
GTCGCCG5100.043.2346451
CGCCGTA5100.043.2346453
ATCTCGG4950.043.13065742
TCTCGGT5000.042.69935243
CTCGGTG5050.042.27658544
CCGTATC5300.042.26350855
CGTATCA5250.041.99936356
GTAGATC5200.041.73013738
TAGATCT5200.041.05706839
TGGCGTA350.001254557439.9993924
GGTGGTC5550.039.72912647
GTATCAT5600.039.374457
Produced by FastQC (version 0.11.5)