FastQCFastQC Report
Wed 25 May 2016
SRR1779898_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779898_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences329121
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT175765.340285183868547No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG47641.4474919558460262No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC27300.8294821661334282No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG21860.664193412149331No Hit
CTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT11660.35427699842914917No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT9230.28044397045463526Illumina PCR Primer Index 3 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT5970.18139225391269473No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC4420.13429711261207883No Hit
CATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT3340.10148243351229486No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACTCGCA207.90771E-570.0223521
TACTCGC207.90771E-570.0223520
AGGGGGG24700.060.08188670
CAACTCG252.390136E-455.9923436
CTCGGAT200.006938885452.51675829
CGTCTTA200.00694723352.50079748
AGACGCA200.00695140952.4928256
CCGTCTT29050.050.23409347
CGTCTTC29450.050.15356448
AAGGGGG29350.050.0859469
GCCGTCT29250.050.01025446
TATGCCG30100.049.76063543
TGCCGTC30050.049.6105245
TCGTATG31250.049.60921540
CGTATGC30550.049.60041841
CTCGTAT31150.049.54378539
TCTCGTA31300.049.4181638
ATCTCGT31350.049.33934437
GTCTTCT29950.049.31627349
ATGCCGT30400.049.26957744
Produced by FastQC (version 0.11.5)