FastQCFastQC Report
Wed 25 May 2016
SRR1779897_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779897_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70961
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2484835.01641746875044No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG20562.8973661588760025No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC2410.33962317329237185No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT2280.32130325108158No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1970.2776172827327687No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GAGCTGC150.002216287270.058
AATGCCC150.002216287270.062
GAGGATG200.006925316552.5000044
CCTTATG200.006925316552.50000415
CCCCTTA200.006925316552.50000413
GTTCTCC200.006925316552.50000461
GGATGCC200.006925316552.50000438
AGCTTTA200.006925316552.5000041
GAGTAAA200.006925316552.50000426
TAAAAGG200.006925316552.50000426
CCAGAAT200.006925316552.50000466
TTATAGG200.006925316552.50000436
CGCTCTA200.006925316552.50000447
AGTTCTC200.006925316552.50000460
TTCGCTC200.006925316552.50000445
GAGATAC200.006925316552.5000041
GCTTTCG200.006925316552.50000442
GAAGGGC200.006925316552.5000046
AGGTCCA200.006925316552.5000045
TCAGAGA305.8419793E-446.6666643