FastQCFastQC Report
Wed 25 May 2016
SRR1779897_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779897_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70961
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1462120.60427558799904No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34354.840687138005383No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG19062.6859824410591737No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC15222.144840123448091No Hit
CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT8901.2542100590465186No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT5440.7666182832823664RNA PCR Primer, Index 34 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT4840.6820647961556348No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC3480.49041022533504325No Hit
CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT2770.3903552655684108No Hit
CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC2440.34385084764870844No Hit
CTAAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT1510.2127929426022745No Hit
TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG1460.20574681867504685No Hit
TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC1210.1705161990389087No Hit
TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT1130.1592424007553445RNA PCR Primer, Index 34 (95% over 22bp)
CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC850.11978410676286974RNA PCR Primer, Index 34 (95% over 21bp)
CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG810.11414720762108763No Hit
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT790.11132875805019658No Hit
CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT740.10428263412296897No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCTTGA150.00221079970.04372426
AGCCGTC150.00221699569.9943645
GGATCTA150.00221699569.9943635
ACCGAGC150.00221699569.9943613
GGCCCTT150.00221699569.9943634
GCCGACA207.88068E-569.9943646
TCTACGA150.00221699569.9943611
GGAACTC207.88068E-569.9943635
CGAATGC207.88068E-569.9943641
ACTCGTA252.8149425E-669.9943638
CTCGAAT207.88068E-569.9943639
AACTCGT252.8149425E-669.9943637
TATAAGC150.00221699569.9943631
GAACTCG353.6488927E-969.99435436
CTAATAC1950.064.610181
CGTATGA650.064.61017641
AGGGGGG18750.064.3948170
GTATGAC600.064.161542
TATGACG550.063.63123743
GACGTCT501.2732926E-1162.99492346
Produced by FastQC (version 0.11.5)