##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779897_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 70961 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.613238257634475 32.0 27.0 32.0 22.0 32.0 2 29.81451783373966 32.0 32.0 32.0 27.0 32.0 3 28.637744676653373 32.0 27.0 32.0 13.0 32.0 4 30.91320584546441 32.0 32.0 32.0 27.0 32.0 5 28.930581587068954 32.0 27.0 32.0 22.0 32.0 6 34.744028409971676 37.0 37.0 37.0 27.0 37.0 7 34.45158608249602 37.0 37.0 37.0 27.0 37.0 8 34.13126928876425 37.0 37.0 37.0 27.0 37.0 9 34.03413142430349 37.0 37.0 37.0 27.0 37.0 10-11 34.87717196770057 37.0 37.0 37.0 29.5 37.0 12-13 34.700053550541845 37.0 37.0 37.0 27.0 37.0 14-15 34.70192781950649 37.0 37.0 37.0 27.0 37.0 16-17 33.53311678245797 37.0 34.5 37.0 24.5 37.0 18-19 34.15174532489677 37.0 37.0 37.0 27.0 37.0 20-21 34.67792872141035 37.0 37.0 37.0 27.0 37.0 22-23 34.463649046659434 37.0 37.0 37.0 27.0 37.0 24-25 34.50159947013148 37.0 37.0 37.0 27.0 37.0 26-27 34.52919913755443 37.0 37.0 37.0 29.5 37.0 28-29 34.37310635419456 37.0 37.0 37.0 27.0 37.0 30-31 34.736637025972016 37.0 37.0 37.0 27.0 37.0 32-33 34.46716506249912 37.0 37.0 37.0 27.0 37.0 34-35 34.18758895731458 37.0 37.0 37.0 27.0 37.0 36-37 34.25340680091881 37.0 37.0 37.0 27.0 37.0 38-39 33.5616958611068 37.0 34.5 37.0 22.0 37.0 40-41 33.63384112399769 37.0 34.5 37.0 24.5 37.0 42-43 34.04606051211229 37.0 37.0 37.0 27.0 37.0 44-45 34.23665111821987 37.0 37.0 37.0 27.0 37.0 46-47 33.67490593424557 37.0 34.5 37.0 24.5 37.0 48-49 33.19312016459746 37.0 32.0 37.0 17.5 37.0 50-51 33.30702075788109 37.0 32.0 37.0 22.0 37.0 52-53 32.865884077169156 37.0 32.0 37.0 17.5 37.0 54-55 32.65898169417004 37.0 32.0 37.0 17.5 37.0 56-57 32.71254632826482 37.0 32.0 37.0 17.5 37.0 58-59 32.87860233085779 37.0 32.0 37.0 17.5 37.0 60-61 33.14919462803512 37.0 32.0 37.0 17.5 37.0 62-63 33.60269021011541 37.0 34.5 37.0 22.0 37.0 64-65 33.69551584673272 37.0 37.0 37.0 22.0 37.0 66-67 33.53585772466566 37.0 34.5 37.0 22.0 37.0 68-69 33.084194134806445 37.0 32.0 37.0 22.0 37.0 70-71 31.210453629458435 37.0 29.5 37.0 13.0 37.0 72-73 32.37080931779428 37.0 32.0 37.0 13.0 37.0 74-75 33.305625625343495 37.0 34.5 37.0 17.5 37.0 76 33.335677343893124 37.0 37.0 37.0 22.0 37.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 11101 1 -1.0537037873584154 11101 2 0.6060623044184226 11101 3 0.4163166354223513 11101 4 -0.28985431822416885 11101 5 0.9481419881686364 11101 6 -0.3033505623977746 11101 7 -1.77005309297963 11101 8 -2.4532673663587765 11101 9 0.5239053076474249 11101 10-11 -0.7620159469151275 11101 12-13 -0.3525218095270688 11101 14-15 0.36276395437170095 11101 16-17 -0.27295898204563684 11101 18-19 -2.3654448501179246 11101 20-21 -1.1689370177546792 11101 22-23 -0.4353274800395184 11101 24-25 -0.2463992509588664 11101 26-27 0.13156220637104354 11101 28-29 -0.027809841633057886 11101 30-31 -1.7318070068539484 11101 32-33 -0.9446964251795009 11101 34-35 -0.7903757570270358 11101 36-37 -0.7617456683316775 11101 38-39 -0.45701091232131574 11101 40-41 -1.5173286232183543 11101 42-43 -0.41473767997612043 11101 44-45 -0.14224335438793645 11101 46-47 -2.3185059139472735 11101 48-49 -0.825080246831817 11101 50-51 -0.7151327398646856 11101 52-53 0.10717559092948648 11101 54-55 -0.44659388921127174 11101 56-57 -1.4803132222005573 11101 58-59 -0.13619272379638403 11101 60-61 -0.5056991313838566 11101 62-63 -1.7526397128713178 11101 64-65 -0.04854794418571373 11101 66-67 -2.4201229638028323 11101 68-69 0.5744486875636596 11101 70-71 0.4518035205316764 11101 72-73 -1.9166302079480957 11101 74-75 -0.6510582092483972 11101 76 -1.2932910553223422 11102 1 -0.15835495014911416 11102 2 -1.190143325936532 11102 3 0.692326427344014 11102 4 -0.6858151505375112 11102 5 -0.05369399714347978 11102 6 -2.2592869149069514 11102 7 0.38600565855035995 11102 8 0.03143275604024609 11102 9 1.0441011460807132 11102 10-11 -2.060975555238258 11102 12-13 -0.9054593860264575 11102 14-15 -0.6681417983822797 11102 16-17 0.8243482394966009 11102 18-19 0.3713972551452329 11102 20-21 -0.1037595391744972 11102 22-23 0.4073897782224165 11102 24-25 -0.6561299731130887 11102 26-27 0.6294202235069122 11102 28-29 -1.794027711890088 11102 30-31 -0.06350835324317927 11102 32-33 1.0739694254936936 11102 34-35 0.4366744265714999 11102 36-37 0.03201198160711982 11102 38-39 -0.10174775442657591 11102 40-41 -1.067512221749567 11102 42-43 -0.9407474718977866 11102 44-45 -1.1661111634454713 11102 46-47 -2.4892525479742034 11102 48-49 0.16390384129547897 11102 50-51 -0.2800042208928346 11102 52-53 -0.1633262450558277 11102 54-55 -2.091942726420573 11102 56-57 -1.261218974954538 11102 58-59 -0.03337754631780143 11102 60-61 -0.3970700004168961 11102 62-63 -1.0148796149521004 11102 64-65 -1.1250473321906185 11102 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-0.751512980393862 21612 9 0.93508951817374 21612 10-11 0.7960980881725988 21612 12-13 -0.12445163408848003 21612 14-15 -0.35927551931251145 21612 16-17 0.10971645655086348 21612 18-19 -0.3994360781880957 21612 20-21 0.6978392980348005 21612 22-23 0.06412427434644741 21612 24-25 1.2069998718481472 21612 26-27 0.02355782040613974 21612 28-29 -0.9735335258435782 21612 30-31 -0.8365219191346469 21612 32-33 0.526246557276643 21612 34-35 1.0122558219203341 21612 36-37 0.5666534544753361 21612 38-39 0.3565855789067598 21612 40-41 -0.5551575705867791 21612 42-43 0.017828109497564526 21612 44-45 0.3254101543839951 21612 46-47 0.9693230334211478 21612 48-49 0.8114329110629228 21612 50-51 0.30405585662654033 21612 52-53 0.8725264681224729 21612 54-55 -0.06117722254460034 21612 56-57 -0.7231860292181054 21612 58-59 -0.10823219748059643 21612 60-61 0.3660113949319381 21612 62-63 -0.0766528707660541 21612 64-65 -0.6751926810278235 21612 66-67 -1.095013314680024 21612 68-69 -1.1640381545416005 21612 70-71 0.5702245731632587 21612 72-73 -0.12222231321125321 21612 74-75 -0.3972204899501577 21612 76 0.3262264885373085 >>END_MODULE >>Per sequence quality scores pass #Quality Count 16 3.0 17 8.0 18 10.0 19 21.0 20 52.0 21 148.0 22 231.0 23 368.0 24 500.0 25 689.0 26 992.0 27 1236.0 28 1652.0 29 2096.0 30 2905.0 31 4074.0 32 5745.0 33 9636.0 34 15998.0 35 18716.0 36 5881.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 29.063851975028538 14.154253745014866 11.369625568974508 45.41226871098209 2 18.22038078326921 16.90130920672501 46.40003382234812 18.47827618765766 3 18.18604585617452 20.984766280069334 42.400755344485 18.428432519271148 4 13.892137934922 38.95238229449979 27.88855850396697 19.266921266611238 5 13.661025070108934 28.918701822127645 46.42550133171742 10.994771776045997 6 22.58032694475761 52.24351747463359 15.155016910935739 10.021138669673055 7 21.007313876636463 24.04137483970068 16.34277983681177 38.60853144685109 8 19.417708318653908 51.86088132918082 13.312946548103888 15.408463804061387 9 19.148546384633814 16.807824016008794 13.064922985865476 50.97870661349192 10-11 19.78128831329885 32.91103563929482 30.119361339327238 17.188314708079087 12-13 20.219557221572412 16.451290145291075 33.11748707036259 30.21166556277392 14-15 19.495215681853413 15.403531517312327 17.433519820746607 47.66773298008765 16-17 34.6190160792548 30.93671171488564 14.691168388269613 19.753103817589942 18-19 35.73089443497132 20.168120516903652 15.428192951057623 28.6727920970674 20-21 20.885415932695427 16.464677780752808 15.091388227336141 47.55851805921563 22-23 19.520719273398583 32.604758985632856 15.025965149625495 32.84855659134307 24-25 34.679970967310034 32.31507515379574 15.268235277537013 17.736718601357207 26-27 35.588633288227335 33.94578822733423 15.734945872801081 14.730632611637349 28-29 20.13502276218799 20.46694197403842 15.98638496990881 43.41165029386478 30-31 18.852355046931816 16.514220480874933 31.576091552273304 33.05733291991994 32-33 19.81819463039955 31.99210767387781 31.203579733633998 16.98611796208865 34-35 48.60583596992524 18.536004453432735 19.90303918598014 12.955120390661884 36-37 39.37162666816984 29.768464367751303 16.397739603444144 14.462169360634714 38-39 20.357661250546073 19.533264751060443 32.0281563112132 28.080917687180285 40-41 18.449830890642616 15.577790304396844 34.61879932356257 31.35357948139797 42-43 33.866967305524234 15.20574971815107 33.17573280721533 17.751550169109358 44-45 19.106269641070448 30.62738687448035 34.994574484576034 15.27176899987317 46-47 33.418827508455465 16.42545095828636 21.684047350620066 28.471674182638107 48-49 32.42696692549429 14.77994954975268 18.82653852115951 33.966545003593524 50-51 19.506489480136977 15.473992756584604 34.494299685742874 30.52521807753555 52-53 15.947492284494299 16.136328405744 49.59414326179169 18.322036047970013 54-55 15.782353189870207 16.054341239307206 37.13059654166491 31.032709029157683 56-57 31.26012880314539 15.50147263990079 22.106509209283974 31.131889347669844 58-59 17.750265996800994 17.088620973935836 45.91915106504415 19.241961964219026 60-61 31.603275038401375 31.02478826397599 21.55197925621116 15.81995744141148 62-63 17.367408628744162 50.607027853524144 15.838388962873712 16.187174554857982 64-65 15.678882727227887 52.78155541619397 14.846706173326663 16.69285568325148 66-67 15.86208840378531 52.848495951859185 14.462678889773601 16.8267367545819 68-69 17.603331407392794 51.99264384661998 13.815335184115218 16.588689561872012 70-71 31.348205352235738 37.957469595975255 13.700483364101407 16.993841687687603 72-73 52.17587373167982 16.798196166854567 13.78523111612176 17.240698985343855 74-75 54.175063771016255 15.116197133475204 13.805544202827063 16.903194892681483 76 54.686509110895024 14.797277300976619 13.79951803153934 16.716695556589016 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 0.5 12 0.0 13 1.5 14 1.5 15 13.0 16 22.0 17 18.0 18 31.5 19 63.5 20 93.0 21 104.0 22 117.5 23 178.0 24 230.0 25 235.0 26 296.5 27 408.5 28 453.0 29 447.0 30 547.0 31 669.5 32 829.0 33 966.0 34 1038.0 35 1115.5 36 1208.5 37 1296.0 38 1435.0 39 1617.5 40 1715.5 41 1955.0 42 2140.0 43 2105.5 44 2201.0 45 2481.0 46 2631.0 47 3074.0 48 5635.0 49 13128.0 50 18503.0 51 10424.0 52 2321.0 53 1927.0 54 1557.0 55 1501.5 56 1317.5 57 1151.5 58 1114.0 59 1034.5 60 917.0 61 780.5 62 682.0 63 662.5 64 550.5 65 425.0 66 366.5 67 341.0 68 301.5 69 258.0 70 220.0 71 186.0 72 162.5 73 116.0 74 84.5 75 76.0 76 66.5 77 46.5 78 36.0 79 36.0 80 31.5 81 21.0 82 16.5 83 18.0 84 12.5 85 8.0 86 7.0 87 5.0 88 5.0 89 7.5 90 7.0 91 3.0 92 2.0 93 2.5 94 4.5 95 8.0 96 10.0 97 19.5 98 70.5 99 1728.0 100 3344.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.002818449570891053 3 0.0 4 0.0 5 0.0 6 0.0014092247854455265 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.046123927227632E-4 24-25 0.00915996110539592 26-27 0.02395682135257395 28-29 0.014092247854455266 30-31 0.009864573498118685 32-33 0.008455348712673158 34-35 0.0063415115345048685 36-37 0.0 38-39 0.0 40-41 0.0014092247854455265 42-43 0.0014092247854455265 44-45 0.0 46-47 0.0014092247854455265 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.002818449570891053 56-57 0.0 58-59 0.0021138371781682895 60-61 0.0 62-63 7.046123927227632E-4 64-65 0.0035230619636138164 66-67 0.0035230619636138164 68-69 0.0 70-71 0.0 72-73 0.0014092247854455265 74-75 0.005636899141782106 76 0.002818449570891053 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 70961.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 34.40481391186708 #Duplication Level Percentage of deduplicated Percentage of total 1 63.31613008929303 21.783796733416946 2 14.594085360858525 10.042135821084822 3 9.53960842139756 9.846253575907893 4 5.9105431309904155 8.134045461591578 5 3.3587285983452118 5.777821620326659 6 1.5646760055705742 3.229943208241146 7 0.7168018350126976 1.7263003621707698 8 0.35225690177766855 0.9695466523865222 9 0.1884164823461948 0.583419061174448 >10 0.36454493323502907 2.0701512098194783 >50 0.036864094372081595 0.8849931652597907 >100 0.03276808388629475 2.6549794957793718 >500 0.008192020971573687 2.020828342328885 >1k 0.01228803145736053 9.671509702512648 >5k 0.0 0.0 >10k+ 0.004096010485786844 20.60427558799904 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT 14621 20.60427558799904 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 3435 4.840687138005383 No Hit TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG 1906 2.6859824410591737 No Hit CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC 1522 2.144840123448091 No Hit CTAATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT 890 1.2542100590465186 No Hit ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT 544 0.7666182832823664 RNA PCR Primer, Index 34 (95% over 23bp) CTTAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT 484 0.6820647961556348 No Hit TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC 348 0.49041022533504325 No Hit CATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT 277 0.3903552655684108 No Hit CTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC 244 0.34385084764870844 No Hit CTAAAACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT 151 0.2127929426022745 No Hit TCATATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG 146 0.20574681867504685 No Hit TTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC 121 0.1705161990389087 No Hit TATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCT 113 0.1592424007553445 RNA PCR Primer, Index 34 (95% over 22bp) CTTTACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTC 85 0.11978410676286974 RNA PCR Primer, Index 34 (95% over 21bp) CTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG 81 0.11414720762108763 No Hit CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATATCGTATGCCGT 79 0.11132875805019658 No Hit CTTATACACATCTCCGAGCCCACGAGACATCATGGGATCTCGTATGCCGT 74 0.10428263412296897 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.005636899141782106 0.0 4 0.0 0.0 0.0 0.005636899141782106 0.0 5 0.0 0.0 0.0 0.009864573498118685 0.0 6 0.0 0.0 0.0 0.009864573498118685 0.0 7 0.0 0.0 0.0 0.018319922210791845 0.0 8 0.0 0.0 0.0 0.01972914699623737 0.0 9 0.0 0.0 0.0 0.03241217006524711 0.0 10 0.0 0.0 0.0 0.03382139485069263 0.0 11 0.0 0.0 0.0 0.03804906920702921 0.0 12 0.0 0.0 0.0 0.0479136427051479 0.0 13 0.0 0.0 0.0 0.049322867490593426 0.0 14 0.0 0.0 0.0 0.060596665774157635 0.0 15 0.0 0.0 0.0 0.06905201448683079 0.0 16 0.0 0.0 0.0 0.06905201448683079 0.0 17 0.0 0.0 0.0 0.06905201448683079 0.0 18 0.0 0.0 0.0 0.07327968884316738 0.0 19 0.0 0.0 0.0 0.08455348712673158 0.0 20 0.0 0.0 0.0 0.08737193669762264 0.0 21 0.0 0.0 0.0 0.0958272854102958 0.0 22 0.0 0.0 0.0 0.1014641845520779 0.0 23 0.0 0.0 0.0 0.12119333154831527 0.0 24 0.0 0.0 0.0 0.1282394554755429 0.0 25 0.0 0.0 0.0 0.13105790504643397 0.0 26 0.0 0.0 0.0 0.13669480418821606 0.0 27 0.0 0.0 0.0 0.1648792998971266 0.0 28 0.0 0.0 0.0 0.1972914699623737 0.0 29 0.0 0.0 0.0 0.2607065853074224 0.0 30 0.0 0.0 0.0 0.33116782457969873 0.0 31 0.0 0.0 0.0 0.4002198390665295 0.0 32 0.0 0.0 0.0 0.4918194501204887 0.0 33 0.0 0.0 0.0 0.6031482081706853 0.0 34 0.0 0.0 0.0 0.7313876636462282 0.0 35 0.0 0.0 0.0 0.9117684361832555 0.0 36 0.0 0.0 0.0 1.324671298318795 0.0 37 0.0 0.0 0.0 1.868632065500768 0.0 38 0.0 0.0 0.0 2.4478234523188793 0.0 39 0.0 0.0 0.0 3.1327066980454052 0.0 40 0.0 0.0 0.0 3.966967771029157 0.0 41 0.0 0.0 0.0 4.775862797874889 0.0 42 0.0 0.0 0.0 5.577711700793394 0.0 43 0.0 0.0 0.0 6.433111145558828 0.0 44 0.0 0.0 0.0 7.339242682600301 0.0 45 0.0 0.0 0.0 8.229872747001874 0.0 46 0.0 0.0 0.0 9.176871802821267 0.0 47 0.0 0.0 0.0 10.11823395949888 0.0 48 0.0 0.0 0.0 11.007454799115006 0.0 49 0.0 0.0 0.0 11.75293471061569 0.0 50 0.0 0.0 0.0 12.54069136567974 0.0 51 0.0 0.0 0.0 13.108608954214287 0.0 52 0.0 0.0 0.0 13.81603979650794 0.0 53 0.0 0.0 0.0 14.448781725172982 0.0 54 0.0 0.0 0.0 15.109708149546934 0.0 55 0.0 0.0 0.0 15.783317596989896 0.0 56 0.0 0.0 0.0 16.40619495215682 0.0 57 0.0 0.0 0.0 16.895195952706416 0.0 58 0.0 0.0 0.0 17.403926100252253 0.0 59 0.0 0.0 0.0 17.922520821296207 0.0 60 0.0 0.0 0.0 18.388974225278673 0.0 61 0.0 0.0 0.0 18.842744606192134 0.0 62 0.0 0.0 0.0 19.334564056312622 0.0 63 0.0 0.0 0.0 19.79538056115331 0.0 64 0.0 0.0 0.0 20.29847380955736 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTCTTGA 15 0.002210799 70.043724 26 AGCCGTC 15 0.002216995 69.99436 45 GGATCTA 15 0.002216995 69.99436 35 ACCGAGC 15 0.002216995 69.99436 13 GGCCCTT 15 0.002216995 69.99436 34 GCCGACA 20 7.88068E-5 69.99436 46 TCTACGA 15 0.002216995 69.99436 11 GGAACTC 20 7.88068E-5 69.99436 35 CGAATGC 20 7.88068E-5 69.99436 41 ACTCGTA 25 2.8149425E-6 69.99436 38 CTCGAAT 20 7.88068E-5 69.99436 39 AACTCGT 25 2.8149425E-6 69.99436 37 TATAAGC 15 0.002216995 69.99436 31 GAACTCG 35 3.6488927E-9 69.994354 36 CTAATAC 195 0.0 64.61018 1 CGTATGA 65 0.0 64.610176 41 AGGGGGG 1875 0.0 64.39481 70 GTATGAC 60 0.0 64.1615 42 TATGACG 55 0.0 63.631237 43 GACGTCT 50 1.2732926E-11 62.994923 46 >>END_MODULE