FastQCFastQC Report
Wed 25 May 2016
SRR1779896_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779896_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215645
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG208509.668668413364557No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG18410.8537179160193836No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC2980.1381900809200306No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG2190.10155579772310973No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGAGA150.002222651269.9983866
ACCCGTC252.3869405E-455.99870725
CGTTACC200.006945053652.49878345
GTTACCG200.006945053652.49878346
CACCCGT200.006945053652.49878324
GCCGTAT3300.047.72616654
CCGTATC3550.046.33695655
TCGGTGG3450.045.65111545
TCGCCGT3400.045.29306852
CTCGGTG3500.044.9989644
CGGTGGT3500.044.9989646
GTAGATC3600.044.72118438
ATCTCGG3550.044.3651742
GATCTCG3600.043.74898541
CGTATCA3700.043.51250556
GTGGTCG3550.043.3792848
AGATCTC3650.043.1496840
TCTCGGT3650.043.1496843
TGGTCGC3600.042.77678349
GTATCAT3700.042.56657857
Produced by FastQC (version 0.11.5)