FastQCFastQC Report
Wed 25 May 2016
SRR1779896_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779896_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences215645
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT115205.342113195297828No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG32121.4894850332722762No Hit
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC15680.7271209626933154No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG14630.6784298267986737No Hit
CTAATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT8450.39184771267592566No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT4600.2133135477289063TruSeq Adapter, Index 10 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT3990.1850263163996383No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC2680.12427832780727585No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACCGGA252.8333343E-669.99930628
ATATCGT401.0617441E-861.24939337
AGGGGGG16800.061.24939370
AATATCG352.9647708E-759.99940536
ATCGTAT352.9647708E-759.99940539
AACTCGT352.9647708E-759.99940537
CGGATAA308.357609E-658.33275231
TTAATAC252.3840505E-456.0124323
CGGACTT252.3867852E-455.9994435
ACCGGAT407.456274E-752.4994829
GTGGGAT200.006944689452.4994821
AGTGCCG200.006944689452.499488
CTGCCGA200.006944689452.4994819
TCTCGGA200.006944689452.4994815
GTGCCGT200.006944689452.499489
AAGGGGG19750.052.2779669
TATGCCG20350.051.25256343
CGTCTTC20000.050.9244948
CGTATGC20550.050.92407241
CCGTCTT19700.050.8116847