FastQCFastQC Report
Wed 25 May 2016
SRR1779895_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779895_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78208
Sequences flagged as poor quality0
Sequence length76
%GC62

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2185827.94854746317512No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG17192.197984860883797No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC2860.36569148936170215No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT1860.23782733224222588No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG1650.21097585924713586No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGTT150.002217137770.014
CGGGGTC150.002217137770.040
TGCGCGT150.002217137770.05
GCAGCTT150.002217137770.030
GCCCCAG252.3772361E-455.99999646
GAGACTA252.3772361E-455.99999626
TGGAGGG200.006927953652.50000424
TGCTTCG200.006927953652.50000452
CAGCACA200.006927953652.50000439
AGGGTTC200.006927953652.50000427
AGAACGC200.006927953652.5000045
GCTTCGC200.006927953652.50000453
TCAGAGT200.006927953652.50000423
GGGCCCT200.006927953652.50000429
AACAGCG200.006927953652.5000047
TACACTG200.006927953652.5000045
GCATCAC200.006927953652.5000047
TTGGAGA305.84567E-446.66666417
GGGTCAG305.84567E-446.66666442
GTAGGGC305.84567E-446.66666433
Produced by FastQC (version 0.11.5)