FastQCFastQC Report
Wed 25 May 2016
SRR1779895_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779895_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences78208
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1244915.9178089198036No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG29673.793729541734861No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG16502.1097585924713584No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC14491.8527516366612113No Hit
CTAATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT8211.0497647299509No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT4410.5638809328968903RNA PCR Primer, Index 14 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT3390.43345949263502453No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3060.3912643207855974No Hit
CATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT2130.27235065466448444No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1980.25317103109656297No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT1280.16366612111292964No Hit
CTAAAACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1070.1368146481178396No Hit
TCATATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG1070.1368146481178396No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT920.11763502454991816No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT810.10356996726677578No Hit
CTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG790.10101268412438626No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACAGTAC150.002217137770.08
CTCGTAA150.002217137770.039
ACACAAT150.002217137770.06
AGGTTCC252.816294E-670.029
AATCTCG252.816294E-670.036
CGGGATA150.002217137770.017
ACGTATG150.002217137770.040
CACGTAT150.002217137770.039
CAGTACA150.002217137770.09
TGCAGGT150.002217137770.015
TTACCGT150.002217137770.032
CTAATGT150.002217137770.022
TAGTACA150.002217137770.016
TGCATGA455.456968E-1269.9999955
TGACGTC501.4551915E-1162.99999645
AGGGGGG17600.062.24431670
GTATGAC453.783498E-1062.22221842
CTGCATG453.783498E-1062.22221854
CTTTATA352.939978E-760.02
GACAGGT308.307594E-658.33333226
Produced by FastQC (version 0.11.5)