FastQCFastQC Report
Wed 25 May 2016
SRR1779894_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779894_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125407
Sequences flagged as poor quality0
Sequence length76
%GC60

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2611920.827386031082796No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG19831.5812514452941222No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT2470.1969587024647747No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC2330.18579505131292512No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG2150.17144178554626138No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAACCGC207.9015925E-569.999213
GTCCGTT150.002220373169.999256
AAGTGCG150.002220373169.999236
TATCAAC252.3829372E-455.9993666
ATGCACC252.3829372E-455.9993635
CAATATC252.3829372E-455.9993663
GTCGCGC200.006937986752.49940531
ACCGGTC200.006937986752.49940527
GAGCTTT200.006937986752.49940534
CGTTGCA200.006937986752.49940559
GACCGGT200.006937986752.49940526
CCGTGGT200.006937986752.49940540
AGTGGCG200.006937986752.4994056
AGCGCCA200.006937986752.49940550
TGCTCGA200.006937986752.49940546
TTAGTAC200.006937986752.49940530
GACGCGT200.006937986752.49940551
CGTCCGT200.006937986752.49940555
GCTCGAC200.006937986752.49940547
TCGACGC200.006937986752.49940549
Produced by FastQC (version 0.11.5)