FastQCFastQC Report
Wed 25 May 2016
SRR1779894_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779894_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences125407
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT85636.828167486663424No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG34772.772572503927213No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT13211.053370226542378No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG7610.6068241804683949No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC6380.5087435310628594No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT4800.3827537537776998No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT4420.3524524149369652No Hit
CTAATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT3470.2766990678351288No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG2490.19855350977218178No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT2240.17861841842959325No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT2010.1602781343944118No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATTCCGT1940.154696308818487No Hit
CTTAAACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT1840.1467222722814516No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT1580.12598977728515953No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCTAAG207.904714E-569.9936240
AGCCGTC301.0175063E-769.99361445
ACCGGTC150.002221075469.99361437
CTGGTAT150.002221075469.99361439
ATGACGA301.0175063E-769.99361444
ATGACCA150.002221075469.99361444
TACTCGC150.002221075469.99361420
ACGTAAT301.0175063E-769.99361447
AGGGGGG16000.060.8069670
ATCTAGG308.341323E-658.32801437
TGACGTA601.8189894E-1258.32801445
ATAAGCG308.341323E-658.32801454
GCATGAA7700.056.35849856
TGACGAA252.3838764E-455.994945
GCCGACA252.3838764E-455.994946
GCTAGGT252.3838764E-455.994950
TAAGCCG1000.055.994943
TGCATGA4200.055.82824755
TAAGCAT3450.055.79201553
AAGCATG2900.055.5121854
Produced by FastQC (version 0.11.5)