FastQCFastQC Report
Wed 25 May 2016
SRR1779893_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779893_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239892
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG217809.079085588514832No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG17630.7349140446534274No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC2550.10629783402531139No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCGGT207.916669E-569.9985446
CTTGCGA150.00222293669.9985435
TTGCGAC150.00222293669.9985436
AGGATCG150.00222293669.998545
TCGGTAT150.00222293669.9985448
CTCACGC200.006940211652.50985327
CTAGATG200.006945937452.498913
TCGTTTA200.006945937452.4989121
TGCGACC200.006945937452.4989137
AGTTCGC200.006945937452.4989110
CGGTATT200.006945937452.4989149
CGTTTAT200.006945937452.4989122
CCTTGTT200.006945937452.4989113
GGTCGAT305.8706535E-446.66569510
GGATCGC305.8706535E-446.6656956
GGGGTGC305.8706535E-446.6656958
AGCAACC503.4698369E-641.99912635
ATCTCGC350.00125391239.99916511
GTCGATG350.00125391239.99916511
TAACACG350.00125391239.9991654
Produced by FastQC (version 0.11.5)