FastQCFastQC Report
Wed 25 May 2016
SRR1779893_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779893_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences239892
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT118324.932219498774448No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30471.2701549030397012No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG16230.676554449502276No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC15490.6457072349223818No Hit
CTAATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT8060.33598452637019993No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT5320.22176646157437513Illumina PCR Primer Index 11 (95% over 22bp)
CTTAAACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT3520.1467326963800377No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC2660.11088323078718756No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTCGCAA150.00222117770.0125125
TCGCAAG150.00222117770.0125126
GTTTGCC150.00222301569.997922
AGCCGTC150.00222301569.9979245
CGGCATG150.00222301569.9979237
AAAAGCG150.00222301569.9979267
ACCGAAG150.00222301569.9979215
GCCGTAT207.917019E-569.9979246
CGTATGA550.063.63446841
AGGGGGG18600.059.6487670
CGTCTAC308.361449E-658.33159648
CCGAAAT252.3875621E-455.99833347
CAACTCG252.3875621E-455.99833336
GGTCGCA200.006940456652.50938424
CACGCTA200.006940456652.50938423
CAGTTGT200.00694618352.498444
CGGTTCA200.00694618352.4984462
AAGCCGT200.00694618352.4984444
CGATCCT200.00694618352.4984456
TCGGTTC200.00694618352.4984461