FastQCFastQC Report
Wed 25 May 2016
SRR1779892_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779892_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319750
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG211756.622361219702892No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG14500.4534792806880375No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTCTA150.002223432370.01
GTTACGA200.006947472752.517
ACGACTT200.006947472752.520
GTACACT350.001254410340.04
TCGCCGT2550.039.8039252
TCGTTGT556.6840566E-638.18181634
GCCGTAT2600.037.6923154
TCGGTGG2850.036.84210645
CGTATCA2800.036.2556
CGCCGTA2800.036.2553
CAAGTTT400.002416831735.019
TGGTCGC2850.034.38596749
GATCTCG3200.033.9062541
GTCGCCG3050.033.27868751
ATCTCGG3300.032.87878842
CTCGGTG3100.032.74193644
CGGTGGT3000.032.66666846
GTAGATC3350.032.38805838
AGATCTC3250.032.30769340
GGTGTAC552.9886598E-431.818181
Produced by FastQC (version 0.11.5)