FastQCFastQC Report
Wed 25 May 2016
SRR1779892_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779892_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences319750
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT112993.5336982017200937No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG30350.9491790461297889No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCC19040.5954652071931197No Hit
TCTTATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCG13970.43690383111806097No Hit
CTAATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT7790.2436278342455043No Hit
ATACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGTCTT5640.17638780297107115TruSeq Adapter, Index 9 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACGGAGTAAGATCTCGTATGCCGT3840.1200938232994527No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGTTAA252.3869956E-456.00675629
CGCGTTA252.3869956E-456.00675628
AAGCCGT252.3888411E-455.99799344
AGGGGGG18800.055.84906870
ATAACTC200.006948454352.4981235
CCGTCTT20850.051.36507447
CGTCTTC21350.051.30964348
TATGCCG21100.051.2540943
CGTATGC21300.051.1014641
GCCGTCT21300.051.1014646
TGCCGTC21100.051.0882245
ATGCCGT21300.050.77283544
TCTCGTA21500.050.62609538
TCGTATG21750.050.5269340
ATCTCGT21300.050.27989237
CTCGTAT21550.050.02141639
GTATGCC21700.049.99821542
CTGCTTG21400.049.88139754
TCTTCTG21700.049.67564450
CTTGAAA22250.049.39149557
Produced by FastQC (version 0.11.5)