FastQCFastQC Report
Wed 25 May 2016
SRR1779891_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779891_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269611
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2766110.25959623309138No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG25310.9387599170656983No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC4120.15281275615609155No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3120.11572228136092369No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3070.11386775762116531No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCGCGA150.002223216269.998722
GCGCTAC150.002223216269.998738
GCTTTCC150.002223216269.998718
CGGCAGC200.00694680452.49902718
TGTCTTG200.00694680452.49902714
GGTGAAT305.871892E-446.66580221
GCCGTAT4250.041.9992254
TGGTCGC4350.041.83830349
AGATCTC4550.040.7684840
GTAGATC4550.040.7684838
TCGGTGG4650.040.64440545
TCGCCGT4400.040.5674352
CGGTGGT4500.040.44369546
GGTAACC350.001254174439.99925619
CTCGGTG4750.039.78873444
GATCTCG4700.039.46735441
GTCGCCG4550.039.23004551
ATCTCGG4800.038.64511542
CGCCGTA4750.038.3150853
TCTCGGT4850.038.24671643
Produced by FastQC (version 0.11.5)