FastQCFastQC Report
Wed 25 May 2016
SRR1779891_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779891_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences269611
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT152805.667424548701648No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG43541.6149192725816084No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC22970.8519682060450056No Hit
TCTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCG21520.7981870175920123No Hit
CTAATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT8920.3308470351728972No Hit
ATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGTCTT5330.19769223065824465TruSeq Adapter, Index 2 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCCGT4180.15503818464380162No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCATCCTGTATCTCGTATGCC3360.12462399531176398No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG20700.059.00856870
TCATGTA252.3879632E-455.99896231
AGCCGTC700.054.99897845
AAGCCGT653.6379788E-1253.8451544
TCTGGTA200.00694170952.50876624
CCTTAGT200.00694680452.49902741
CCGCTTT200.00694680452.49902767
GCGTCGG200.00694680452.49902713
TCTTAAG552.2755557E-950.90814650
CTTAAGT352.0834144E-549.99907346
GGGTCTA352.0834144E-549.9990731
CGTCTAC352.0834144E-549.99907348
GCCGTCT24550.049.18442546
CCGTCTT24300.049.11431547
GTCTTCT22700.048.7215649
CGTATGC26350.048.613941
CGTCTTC23650.048.5403348
TCTTCTG22750.048.4606450
TATGCCG26650.048.4606443
CGTCTTA1300.048.46063648