FastQCFastQC Report
Wed 25 May 2016
SRR1779890_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779890_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133848
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2436318.201990317374932No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG23631.7654354192815733No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3130.23384734923196462No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3130.23384734923196462No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG2660.19873289104058336No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAAAAG150.002220598370.0000125
CATGACA150.002220598370.0000131
AACGGCC150.002220598370.0000137
CAAGGTA150.002220598370.0000144
TGTTCGA150.002220598370.0000137
GTAGCCG308.339859E-658.33333625
CCAAGAG252.3833827E-456.00000461
TTTAACG200.00693868752.534
GCAGTTA200.00693868752.565
AAGGTCC200.00693868752.561
TAACGGC200.00693868752.536
CCAGATA200.00693868752.516
AGACGTA200.00693868752.55
CATGTAA200.00693868752.533
GCTAATC200.00693868752.554
CGATATG200.00693868752.516
TAAGAAC200.00693868752.549
TGCACCC200.00693868752.55
CCGTTGA352.0775064E-550.029
TTAGAGT305.8606936E-446.6666684
Produced by FastQC (version 0.11.5)