FastQCFastQC Report
Wed 25 May 2016
SRR1779890_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779890_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences133848
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1379710.307961269499732No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40813.048980933596318No Hit
TCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG18821.4060725599187136No Hit
CTCTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC17671.3201542047695893No Hit
CTAATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT9840.7351622736238121RNA PCR Primer, Index 19 (95% over 21bp)
ATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTCTT5520.41240810471579703No Hit
CTTAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT4660.3481561173868866No Hit
CTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGTC2710.20246847169924093RNA PCR Primer, Index 19 (95% over 22bp)
CATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT2670.19948000717231487RNA PCR Primer, Index 19 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCC2400.1793078716155639No Hit
CTAAAACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCGT1820.13597513597513597RNA PCR Primer, Index 19 (95% over 21bp)
TCATATACACATCTCCGAGCCCACGAGACGCGTTAAAATCTCGTATGCCG1650.1232741617357002No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGT207.890332E-570.02280422
AATCACG150.002221021369.9966436
ACCGATT150.002221021369.9966441
GGTCCAC150.002221021369.9966449
GAGTTTC150.002221021369.9966411
CACCGAT150.002221021369.9966440
AGGGGGG19500.063.53540870
CGTCTTA352.9569674E-759.9971248
TATGCAG352.9569674E-759.9971243
CGAATGC352.9569674E-759.9971241
GACGCGA308.323757E-658.35233726
ACGCGTA308.323757E-658.35233727
CCCAACG252.379547E-456.01824621
CCTTCAA252.3839484E-455.9973110
ATCTCGA252.3839484E-455.9973137
TACTTAA252.3839484E-455.9973136
GCAGTCT252.3839484E-455.9973146
CGCAAAG252.3839484E-455.9973112
ATCTACG452.6804628E-854.4418310
TCTACGA452.6804628E-854.4418311
Produced by FastQC (version 0.11.5)