FastQCFastQC Report
Wed 25 May 2016
SRR1779889_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779889_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236892
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3259613.759856812387081No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG29351.2389612143930568No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3890.164209850902521No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3150.13297198723468923No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3070.1295949208922209No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCTAT150.002222718470.01
GTTCGCA252.3871502E-455.99999635
CGACTTG200.006945259452.558
AACGTTA305.8699027E-446.6666648
ATCGTCA305.8699027E-446.66666414
CGAAGCA305.8699027E-446.66666416
TCTCGGT5200.044.42307743
TCGGTGG5250.044.045
ATCTCGG5200.043.7542
GTCGCCG5400.043.42592651
AGATCTC5250.043.33333240
CTCGGTG5250.043.33333244
GCCGTAT5100.043.2352954
GATCTCG5350.043.1775741
TGGTCGC5450.043.02752349
CGGTGGT5550.042.88288546
TCGCCGT5250.042.66666852
TAGATCT5500.041.36363639
CGTATCA5500.040.72727256
CCGTATC5450.040.45871755