FastQCFastQC Report
Wed 25 May 2016
SRR1779889_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779889_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences236892
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT183177.732215524373977No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG50362.125863262583793No Hit
TCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCG23450.9899025716360197No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC20710.8742380494064805No Hit
CTAATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT12270.5179575502760752No Hit
ATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTCTT7690.3246205021697651No Hit
CTTAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT6150.2596119750772504No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCC4320.1823615824932881No Hit
CATATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT3640.15365651858230753No Hit
CTATACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGTC3100.13086132077064655No Hit
CTTATACACATCTCCGAGCCCACGAGACACAAATAAATCTCGTATGCCGT3020.12748425442817823No Hit
CTAAAACACATCTCCGAGCCCACGAGACCCAAATAAATCTCGTATGCCGT2530.10679972308055992No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATGCTAT150.00222120370.0120432
AATCACG150.00222306469.9972536
AGGGGGG28550.059.69993270
AATATCG553.6379788E-1157.2704836
CGTCTCA252.3876126E-455.99780312
CCGTCTC252.3876126E-455.99780311
AAGCCGT252.3876126E-455.99780344
AACTCGT509.767973E-1055.99780337
ACTCCGT252.3876126E-455.9978038
CGGTATG252.3876126E-455.99780343
GACTCCG252.3876126E-455.9978037
CGTATGA608.367351E-1152.4979441
GATCATA200.00694633652.497941
AGAACGC200.00694633652.4979418
ATATCGT608.367351E-1152.4979437
TGCGAGT200.00694633652.4979413
GAACGCC200.00694633652.4979419
ATCGCGT200.00694633652.4979437
GCGAGTA200.00694633652.4979414
AAGGGGG32950.051.62164769
Produced by FastQC (version 0.11.5)