FastQCFastQC Report
Wed 25 May 2016
SRR1779888_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779888_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228418
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2750912.04327154602527No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG23031.0082392806171143No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3590.15716799901934173No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG2760.12083110788116525No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTTC150.002222864769.99802435
ACGGTCA252.387301E-455.9984211
TGGTCGC3700.052.9714849
CGACTAT200.006939702752.5100269
GTCTGAC200.006945715852.498521
CCGGCTC200.006945715852.4985226
TCTCGGT3800.052.4985243
ACGACGA200.006945715852.4985264
CGGTGGT3750.052.26519846
TCGGTGG3750.052.26519845
GCCGTAT3750.052.26519854
TCGCCGT3800.051.57749652
GATCTCG3800.051.57749641
GTCGCCG3850.050.90765451
AGATCTC3850.050.90765440
CTCGGTG3850.050.90765444
CGTATCA3900.050.25499756
GTAGATC3900.050.25499738
CGCCGTA3900.050.25499753
TTTGGCC352.0798258E-550.0095414
Produced by FastQC (version 0.11.5)