FastQCFastQC Report
Wed 25 May 2016
SRR1779888_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779888_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences228418
Sequences flagged as poor quality0
Sequence length76
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT156566.854100815172185No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG40161.758180178444781No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC22450.9828472362073041No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCG20070.8786522953532558No Hit
CTAATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT9070.39707903930513355No Hit
ATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGTCTT5410.23684648320184926TruSeq Adapter, Index 7 (95% over 21bp)
CTTAAACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT4850.2123300265303085No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCC3590.15716799901934173No Hit
CATATACACATCTCCGAGCCCACGAGACCTTGGGTCATCTCGTATGCCGT2610.11426419984414539No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACGTCA207.921912E-569.9877446
CCGTATA150.002224160669.987742
AGGGGGG19950.061.2307570
CGAGAAC252.3838702E-456.01471723
CGTCTAC252.3864518E-456.0024548
TACGACA252.3864518E-456.0024549
GCCGACT200.00694975452.49080746
CGCTATG200.00694975452.49080710
CGGTATG200.00694975452.49080743
AACTTGG352.0789395E-550.01313827
CGTATTC352.0816355E-550.0021948
AAGGGGG24550.049.61520869
CTGCTTG23100.049.54762354
CGTATGC25000.049.2713741
TATGCCG24950.049.22985543
CCGTCTT23500.048.9914247
CTTGAAA24650.048.9879457
TCGTATG25650.048.9777740
GCCGTCT23750.048.9177546
GTATGCC25200.048.8803342