FastQCFastQC Report
Wed 25 May 2016
SRR1779887_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779887_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58428
Sequences flagged as poor quality0
Sequence length76
%GC81

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3440158.87759293489423No Hit
CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG34965.983432600807832No Hit
TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT3610.617854453344287No Hit
ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC3490.5973163551721777No Hit
CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG3030.5185869788457589No Hit
GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG960.1643047853768741No Hit
TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG940.1608817690148559No Hit
CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG860.14718970356678304No Hit
TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG610.10440199904155542No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGGGGG452.6446287E-854.44444770
AAAAAGG3450.052.7536270
TTGGGGG200.00691920752.52
TCTTTAT200.00691920752.51
CATGCAA200.00691920752.510
TCGGTGG5550.046.03603445
TCTCGGT5550.046.03603443
CTCGGTG5550.046.03603444
ATCTCGG5600.045.62542
TAAAAAA5600.045.62564
GTATCAT5450.045.5963357
CCGTATC5250.045.33333255
CGTATCA5250.045.33333256
GATCTCG5650.045.2212441
CGGTGGT5650.045.2212446
AGATCTC5650.045.2212440
TAGATCT5650.045.2212439
CATTAAA5500.045.1818261
TCGCCGT5350.045.14018652
GCCGTAT5350.045.14018654