##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779887_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 58428 Sequences flagged as poor quality 0 Sequence length 76 %GC 81 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 28.922075032518656 32.0 27.0 32.0 22.0 32.0 2 29.723351817621687 32.0 32.0 32.0 22.0 32.0 3 29.61093996029301 32.0 32.0 32.0 22.0 32.0 4 29.032655576093653 32.0 32.0 32.0 13.0 32.0 5 29.771633463407955 32.0 32.0 32.0 22.0 32.0 6 34.061391798452796 37.0 37.0 37.0 27.0 37.0 7 33.798469911686176 37.0 37.0 37.0 22.0 37.0 8 34.025433011569795 37.0 37.0 37.0 27.0 37.0 9 34.18876223728349 37.0 37.0 37.0 27.0 37.0 10-11 34.00310638734853 37.0 37.0 37.0 27.0 37.0 12-13 33.802397822961595 37.0 37.0 37.0 22.0 37.0 14-15 34.17922057917437 37.0 37.0 37.0 27.0 37.0 16-17 34.46657424522489 37.0 37.0 37.0 29.5 37.0 18-19 34.112540220442256 37.0 37.0 37.0 24.5 37.0 20-21 34.451803929622784 37.0 37.0 37.0 29.5 37.0 22-23 34.52304545765729 37.0 37.0 37.0 29.5 37.0 24-25 34.21594954473882 37.0 37.0 37.0 27.0 37.0 26-27 34.48398028342575 37.0 37.0 37.0 29.5 37.0 28-29 34.35512083247758 37.0 37.0 37.0 27.0 37.0 30-31 33.86883001300747 37.0 37.0 37.0 24.5 37.0 32-33 34.21970630519614 37.0 37.0 37.0 27.0 37.0 34-35 34.34215615800643 37.0 37.0 37.0 27.0 37.0 36-37 34.903231327445745 37.0 37.0 37.0 32.0 37.0 38-39 35.047853768741014 37.0 37.0 37.0 32.0 37.0 40-41 34.66409940439515 37.0 37.0 37.0 29.5 37.0 42-43 34.57353494899706 37.0 37.0 37.0 27.0 37.0 44-45 34.45435407681249 37.0 37.0 37.0 27.0 37.0 46-47 34.635560005476826 37.0 37.0 37.0 29.5 37.0 48-49 35.02050386800849 37.0 37.0 37.0 32.0 37.0 50-51 35.23913192305059 37.0 37.0 37.0 32.0 37.0 52-53 34.610186896693364 37.0 37.0 37.0 29.5 37.0 54-55 34.71923564044636 37.0 37.0 37.0 29.5 37.0 56-57 34.6044704593688 37.0 37.0 37.0 29.5 37.0 58-59 34.35945950571644 37.0 37.0 37.0 27.0 37.0 60-61 34.228563360032865 37.0 37.0 37.0 27.0 37.0 62-63 33.84571609502294 37.0 37.0 37.0 22.0 37.0 64-65 33.67553227904429 37.0 37.0 37.0 22.0 37.0 66-67 33.83330766071062 37.0 37.0 37.0 22.0 37.0 68-69 34.092370096529066 37.0 37.0 37.0 27.0 37.0 70-71 34.123510987882526 37.0 37.0 37.0 27.0 37.0 72-73 34.184988361744374 37.0 37.0 37.0 27.0 37.0 74-75 33.70962552201 37.0 37.0 37.0 20.0 37.0 76 34.05894434175396 37.0 37.0 37.0 27.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 0.4331967026270931 11101 2 -0.13717600360484639 11101 3 -0.3669028103248628 11101 4 -0.24402865572001176 11101 5 0.19248186756399122 11101 6 -1.5604707915453986 11101 7 -1.1187537628525277 11101 8 0.9683008322656406 11101 9 -0.18635539450959726 11101 10-11 -0.16548483909718215 11101 12-13 -0.7156500584881158 11101 14-15 0.4606751295330085 11101 16-17 0.3816032068797952 11101 18-19 0.30089286589638675 11101 20-21 0.46684290543373663 11101 22-23 -0.4789000050093506 11101 24-25 0.525624008048176 11101 26-27 -0.36198226899382746 11101 28-29 0.365517033634859 11101 30-31 0.6590481777198391 11101 32-33 -0.024568449358262967 11101 34-35 0.4515232387543193 11101 36-37 -0.38730358203650894 11101 38-39 -0.2656483000547212 11101 40-41 -1.0774806187369919 11101 42-43 -0.9919688955440975 11101 44-45 -1.4382790129033722 11101 46-47 -0.3968027083046124 11101 48-49 -0.749083901560077 11101 50-51 0.08755562585991328 11101 52-53 -0.07777115080573793 11101 54-55 0.9809089274113063 11101 56-57 -0.252268230005086 11101 58-59 -0.27300671522895925 11101 60-61 0.03672900159813963 11101 62-63 0.3715222195377592 11101 64-65 -0.5277191331831759 11101 66-67 -2.7233402357853116 11101 68-69 0.18068826942634786 11101 70-71 0.6451245587952954 11101 72-73 -0.7088008946258526 11101 74-75 0.18331325209794613 11101 76 0.305541444278866 11102 1 -1.4398683128527807 11102 2 0.27768467750970416 11102 3 0.7724160751240525 11102 4 -1.192945064388745 11102 5 -1.7161868630861647 11102 6 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21612 38-39 0.47626346465116143 21612 40-41 -0.10395120697229032 21612 42-43 -1.4684394837793846 21612 44-45 -0.17724960113866217 21612 46-47 -0.027685061245783515 21612 48-49 0.24282786314580562 21612 50-51 -0.01685613884597359 21612 52-53 -0.4792417390410222 21612 54-55 0.8948795156466005 21612 56-57 0.46832000528902995 21612 58-59 -0.12447730346424635 21612 60-61 0.8933466486569657 21612 62-63 0.09358104306717507 21612 64-65 -0.10271913318317871 21612 66-67 -0.1395167063735414 21612 68-69 -0.6126940835148247 21612 70-71 0.6252716176188216 21612 72-73 0.008110870080031418 21612 74-75 -1.076245571431457 21612 76 -0.7341644380740746 >>END_MODULE >>Per sequence quality scores pass #Quality Count 11 6.0 12 17.0 13 38.0 14 64.0 15 118.0 16 189.0 17 274.0 18 318.0 19 332.0 20 338.0 21 412.0 22 449.0 23 520.0 24 611.0 25 641.0 26 790.0 27 802.0 28 933.0 29 1109.0 30 1260.0 31 1581.0 32 2035.0 33 3016.0 34 5090.0 35 9349.0 36 28136.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 74.90244403368249 5.841377421784076 3.9518723899500237 15.304306154583418 2 68.85739713835832 6.4558088587663445 17.363250496337372 7.323543506537961 3 68.71020743479154 6.911070034914767 16.998699253782434 7.380023276511262 4 68.0632573423701 12.08838228246731 10.376874101458206 9.471486273704388 5 67.5172862326282 10.077360169781612 17.419730266310673 4.985623331279523 6 72.08187855137948 16.478400766755666 5.914972273567468 5.524748408297392 7 70.80680495652769 9.707674402683644 6.091257616211405 13.394263024577258 8 71.17991373998768 16.2781543095776 5.841377421784076 6.700554528650647 9 69.88943657150682 5.997124666255905 5.24063805024988 18.872800711987402 10-11 71.20900937906484 11.108543848839597 10.643869377695625 7.038577394399945 12-13 70.34897651810775 7.064250017115081 11.335318682823303 11.251454781953859 14-15 75.25843773533238 9.425275552817142 6.786985691791607 8.529301020058876 16-17 74.71845690422401 7.891764222632984 6.048469911686178 11.341308961456836 18-19 70.65020195796536 7.253371671116588 10.042274252070925 12.054152118847128 20-21 74.6942792349493 7.169569124128193 6.937657781010655 11.198493859911856 22-23 74.69684651919046 6.81956270591759 5.998887510162167 12.484703264729793 24-25 71.68737591565689 11.318203601013213 6.040768124871637 10.953652358458275 26-27 74.67566919969877 11.099986307934552 6.497740809201067 7.726603683165606 28-29 71.68346825953755 12.050079586492545 9.757475139918188 6.508977014051723 30-31 74.68679400287533 11.49277743547614 7.217429999315398 6.602998562333127 32-33 75.82111010320571 11.10103205709689 6.332688654218084 6.745169185479316 34-35 75.60353606668208 11.102838609192746 6.815400545985127 6.4782247781400555 36-37 75.64653328084619 7.614630222328718 10.632926557926986 6.105909938898113 38-39 75.22677483398371 6.985520640788663 11.669062778120079 6.118641747107551 40-41 74.8314164441706 10.112446087492298 8.368419251044019 6.687718217293079 42-43 71.24323954268502 10.2630588074211 11.438009173683849 7.055692476210036 44-45 70.65704799068939 6.534538235092764 12.151708085164648 10.656705689053194 46-47 73.77199288012596 6.1289107961936065 8.810844115834874 11.288252207845554 48-49 75.44584788115287 6.218764975696583 11.514171287738757 6.821215855411788 50-51 78.18340521667693 6.1408913534606695 8.499349626891217 7.176353802971177 52-53 70.74433490792086 6.9350311494488945 11.49791196001917 10.822721982611077 54-55 73.55012622480852 6.732275041718369 8.133156475974499 11.584442257498608 56-57 73.55503448748011 7.253495815290875 7.346774607630034 11.844695089598986 58-59 70.53074777072243 10.867407191880467 10.952984065586117 7.6488609718109775 60-61 69.36005613842914 7.711332089616102 12.328204426035908 10.600407345918839 62-63 69.9727865541616 11.427079945915414 10.881955260410427 7.718178239512555 64-65 69.98870404600534 11.589477647703156 11.420894091873759 7.000924214417744 66-67 69.87548671430405 16.055795644174403 6.864062299430919 7.204655342090625 68-69 70.18159412600339 16.809865302000787 6.090506101631095 6.918034470364728 70-71 70.36241495870951 17.05532497539686 6.063925377604724 6.518334688288905 72-73 71.31181912317184 16.40779440835922 5.671227953069241 6.609158515399691 74-75 74.56439134602219 13.706695878406133 5.740791455566206 5.988121320005478 76 79.94214904323418 8.261732790196145 5.942559819258549 5.853558347311129 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.5 4 1.0 5 0.5 6 0.0 7 0.0 8 0.0 9 0.5 10 1.0 11 2.0 12 3.0 13 3.0 14 2.5 15 3.0 16 6.5 17 9.0 18 12.5 19 20.0 20 28.0 21 32.0 22 37.0 23 63.0 24 82.0 25 80.0 26 103.0 27 133.5 28 178.0 29 215.0 30 229.5 31 251.5 32 290.0 33 321.0 34 342.0 35 363.5 36 384.5 37 405.0 38 391.0 39 437.5 40 536.0 41 706.5 42 839.0 43 1604.0 44 2782.0 45 2051.0 46 907.0 47 875.0 48 757.0 49 636.0 50 601.0 51 563.0 52 552.0 53 626.5 54 674.0 55 646.0 56 626.0 57 605.5 58 577.0 59 553.5 60 513.0 61 461.0 62 426.0 63 393.5 64 314.0 65 242.5 66 218.5 67 219.0 68 208.0 69 180.5 70 156.0 71 148.0 72 120.5 73 91.0 74 83.5 75 78.0 76 76.0 77 67.5 78 57.5 79 54.0 80 48.0 81 48.0 82 48.0 83 42.0 84 56.5 85 67.5 86 74.0 87 84.0 88 77.5 89 81.0 90 97.5 91 131.5 92 159.0 93 169.0 94 195.5 95 266.0 96 320.0 97 399.0 98 727.0 99 17880.5 100 34785.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 8.557540905045525E-4 22-23 8.557540905045525E-4 24-25 0.0 26-27 0.0 28-29 0.001711508181009105 30-31 0.0 32-33 0.001711508181009105 34-35 0.0025672622715136578 36-37 0.001711508181009105 38-39 0.0 40-41 0.0 42-43 0.0 44-45 0.0 46-47 0.0 48-49 0.0 50-51 0.0 52-53 0.0 54-55 8.557540905045525E-4 56-57 0.001711508181009105 58-59 0.001711508181009105 60-61 0.001711508181009105 62-63 0.001711508181009105 64-65 0.0 66-67 8.557540905045525E-4 68-69 0.001711508181009105 70-71 8.557540905045525E-4 72-73 0.0025672622715136578 74-75 0.00684603272403642 76 0.00342301636201821 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 58428.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 17.99650852331074 #Duplication Level Percentage of deduplicated Percentage of total 1 70.56585829766999 12.699390703087559 2 9.320019020446981 3.354556034777846 3 7.123157394198763 3.8457588827274596 4 5.611031859248692 4.039159307181488 5 3.3095577746077036 2.978024234955843 6 1.7403708987161197 1.8792359827479974 7 1.0746552543984784 1.3538029711782023 8 0.5040418449833571 0.7256794687478606 9 0.19971469329529246 0.32347504621072093 >10 0.456490727532097 1.5369343465461764 >50 0.0475511174512601 0.6691996987745601 >100 0.028530670470756064 1.7337577873622236 >500 0.0 0.0 >1k 0.009510223490252021 5.983432600807832 >5k 0.0 0.0 >10k+ 0.009510223490252021 58.87759293489423 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 34401 58.87759293489423 No Hit CTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTG 3496 5.983432600807832 No Hit TCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGT 361 0.617854453344287 No Hit ATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGGTC 349 0.5973163551721777 No Hit CTCTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 303 0.5185869788457589 No Hit GGTGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 96 0.1643047853768741 No Hit TCTTTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGG 94 0.1608817690148559 No Hit CTATACACATCTGACGCTGCCGACGAATAGAGAGGTGTAGATCTCGGTGG 86 0.14718970356678304 No Hit TGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 61 0.10440199904155542 No Hit >>END_MODULE >>Adapter Content warn #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.010269049086054631 0.0 2 0.0 0.0 0.0 0.010269049086054631 0.0 3 0.0 0.0 0.0 0.01711508181009105 0.0 4 0.0 0.0 0.0 0.01711508181009105 0.0 5 0.0 0.0 0.0 0.02909563907715479 0.0 6 0.0 0.0 0.0 0.02909563907715479 0.0 7 0.0 0.0 0.0 0.02909563907715479 0.0 8 0.0 0.0 0.0 0.02909563907715479 0.0 9 0.0 0.0 0.0 0.02909563907715479 0.0 10 0.0 0.0 0.0 0.041076196344218524 0.0 11 0.0 0.0 0.0 0.051345245430273155 0.0 12 0.0 0.0 0.0 0.05990278633531868 0.0 13 0.0 0.0 0.0 0.061614294516327786 0.0 14 0.0 0.0 0.0 0.08215239268843705 0.0 15 0.0 0.0 0.0 0.08215239268843705 0.0 16 0.0 0.0 0.0 0.08557540905045526 0.0 17 0.0 0.0 0.0 0.09070993359348258 0.0 18 0.0 0.0 0.0 0.09070993359348258 0.0 19 0.0 0.0 0.0 0.09413294995550078 0.0 20 0.0 0.0 0.0 0.0992674744985281 0.0 21 0.0 0.0 0.0 0.11638255630861916 0.0 22 0.0 0.0 0.0 0.15574724447182858 0.0 23 0.0 0.0 0.0 0.1711508181009105 0.0 24 0.0 0.0 0.0 0.21565003080714726 0.0 25 0.0 0.0 0.0 0.22420757171219277 0.0 26 0.0 0.0 0.0 0.2618607516943931 0.0 27 0.0 0.0 0.0 0.2875333744095297 0.0 28 0.0 0.0 0.0 0.3029369480386116 0.0 29 0.0 0.0 0.0 0.3457246525638393 0.0 30 0.0 0.0 0.0 0.3594167180119121 0.0 31 0.0 0.0 0.0 0.41589648798521256 0.0 32 0.0 0.0 0.0 0.45354966796741286 0.0 33 0.0 0.0 0.0 0.5699322242760321 0.0 34 0.0 0.0 0.0 0.6777572396796057 0.0 35 0.0 0.0 0.0 0.8061203532552885 0.0 36 0.0 0.0 0.0 0.9687136304511536 0.0 37 0.0 0.0 0.0 1.2596700212227014 0.0 38 0.0 0.0 0.0 1.5968371328814952 0.0 39 0.0 0.0 0.0 1.9562538508934073 0.0 40 0.0 0.0 0.0 2.324228109810365 0.0 41 0.0 0.0 0.0 2.62031902512494 0.0 42 0.0 0.0 0.0 3.1902512494009723 0.0 43 0.0 0.0 0.0 3.647223933730403 0.0 44 0.0 0.0 0.0 4.212021633463408 0.0 45 0.0 0.0 0.0 4.6108030396385296 0.0 46 0.0 0.0 0.0 5.06948723214897 0.0 47 0.0 0.0 0.0 5.435749982884918 0.0 48 0.0 0.0 0.0 5.750667488190594 0.0 49 0.0 0.0 0.0 6.070719518039296 0.0 50 0.0 0.0 0.0 6.413021154241117 0.0 51 0.0 0.0 0.0 6.6714588895734925 0.0 52 0.0 0.0 0.0 6.979530362155131 0.0 53 0.0 0.0 0.0 7.234545081125487 0.0 54 0.0 0.0 0.0 7.386869309235298 0.0 55 0.0 0.0 0.0 7.684671732730883 0.0 56 0.0 0.0 0.0 7.955090025330321 0.0 57 0.0 0.0 0.0 8.175874580680496 0.0 58 0.0 0.0 0.0 8.364140480591498 0.0 59 0.0 0.0 0.0 8.562675429588554 0.0 60 0.0 0.0 0.0 8.768056411309646 0.0 61 0.0 0.0 0.0 9.053878277538166 0.0 62 0.0 0.0 0.0 9.281508865612377 0.0 63 0.0 0.0 0.0 9.450948175532279 0.0 64 0.0 0.0 0.0 9.574176764564935 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position AAGGGGG 45 2.6446287E-8 54.444447 70 AAAAAGG 345 0.0 52.75362 70 TTGGGGG 20 0.006919207 52.5 2 TCTTTAT 20 0.006919207 52.5 1 CATGCAA 20 0.006919207 52.5 10 TCGGTGG 555 0.0 46.036034 45 TCTCGGT 555 0.0 46.036034 43 CTCGGTG 555 0.0 46.036034 44 ATCTCGG 560 0.0 45.625 42 TAAAAAA 560 0.0 45.625 64 GTATCAT 545 0.0 45.59633 57 CCGTATC 525 0.0 45.333332 55 CGTATCA 525 0.0 45.333332 56 GATCTCG 565 0.0 45.22124 41 CGGTGGT 565 0.0 45.22124 46 AGATCTC 565 0.0 45.22124 40 TAGATCT 565 0.0 45.22124 39 CATTAAA 550 0.0 45.18182 61 TCGCCGT 535 0.0 45.140186 52 GCCGTAT 535 0.0 45.140186 54 >>END_MODULE