FastQCFastQC Report
Wed 25 May 2016
SRR1779887_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779887_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences58428
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT2033034.79496131991511No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG57589.854864106250428No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG28834.93427808584925No Hit
CTCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC19033.2570000684603273No Hit
CTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT14362.457725747929075No Hit
ATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCTT8021.3726295611693025RNA PCR Primer, Index 19 (95% over 23bp)
CTTAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT7101.2151708085164648No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC4770.8163894023413433No Hit
CATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3970.6794687478606147No Hit
CTAAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT3200.5476826179229136No Hit
CTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC2950.504894913397686No Hit
TCATATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG2450.41931950434723075No Hit
TTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC1470.25159170260833846No Hit
CAAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT1430.24474566988430205No Hit
TATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTCT1340.22934209625522012RNA PCR Primer, Index 19 (95% over 22bp)
CTTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG1330.227630588074211No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC1050.17970835900595605No Hit
CTTTACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGTC1030.17628534264393783RNA PCR Primer, Index 19 (95% over 21bp)
CTTATACACATCTCCGAGCCCACGAGACTAATGAAAATCTCGTATGCCGT930.15917026083384678No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGACGT840.14376668720476485No Hit
CTTATACACATCTCCGAGCCCACGAGACTTATGAAATCTCGTATGCCGTC780.1334976381187102No Hit
CATAAACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCGT740.12665160539467377No Hit
CTTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG730.1249400972136647No Hit
CCTTATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCCG660.11295953994660095No Hit
CTTATACACATCTCCGAGCCAACGAGACTTATGAAAATCTCGTATGCCGT610.10440199904155542No Hit
CTCTAATACACATCTCCGAGCCCACGAGACTTATGAAAATCTCGTATGCC590.1009789826795372No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA252.8073482E-670.0000137
CTTTACA252.8073482E-670.000011
CACCGAG252.8073482E-670.0000112
TTACACA252.8073482E-670.000013
CAGCCCA150.002214316370.016
TTATGCT150.002214316370.052
ACTCGTA150.002214316370.038
ACGCTGT150.002214316370.039
ACTCGAA150.002214316370.038
AATCTCC401.3096724E-1070.09
AACGAGC150.002214316370.013
ACGTCAT150.002214316370.047
CCGTCTA301.0073563E-770.047
ATGAACC150.002214316370.039
CAACACC150.002214316370.09
TCTACTG353.6325218E-970.050
TCAACGA207.865872E-570.011
ACATCAA207.865872E-570.08
ACCGAGC207.865872E-570.013
AACTCGT150.002214316370.037
Produced by FastQC (version 0.11.5)