FastQCFastQC Report
Wed 25 May 2016
SRR1779886_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779886_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96352
Sequences flagged as poor quality0
Sequence length76
%GC65

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG3256533.79794918631684No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG76087.8960478246429755No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC9851.022293258053803No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG8060.8365161076054468No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT8040.8344403852540684No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2400.24908668216539354No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1740.1805878445699103No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT1390.14426270342079045No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGACCTA150.002218705870.0000150
GAATATA150.002218705870.0000127
CGCTAAT150.002218705870.0000126
ACCAGCA150.002218705870.000012
AACGACC150.002218705870.0000110
TGGTCTA150.002218705870.0000135
TTTGAGC252.3800206E-456.0000048
GCACTTA252.3800206E-456.00000432
CTTTATA608.367351E-1152.5000042
AAGTAAC200.006932819352.530
TACCAGC200.006932819352.51
TTTGTGA200.006932819352.531
CAGCAGA200.006932819352.54
CAATTTG200.006932819352.536
TAAGAGC200.006932819352.537
GCGAGGA200.006932819352.542
AGAAATG305.8524765E-446.66666829
GGAACTG305.8524765E-446.66666836
CGCCGTA12500.043.453
GCCGTAT12450.043.29317554