FastQCFastQC Report
Wed 25 May 2016
SRR1779886_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779886_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences96352
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT1385414.378528727997342No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1267213.151776818332781No Hit
CTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGT83628.678595151112587No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCG21342.2147957489206243No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC11591.2028811026237132No Hit
ATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCTT8780.9112421122550648Illumina PCR Primer Index 3 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC5400.5604450348721355No Hit
TCTTTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC4380.4545831949518433No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGACGT2290.237670209232813No Hit
CTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC1960.2034207904350714No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGAATCTCGTATGCCGT1560.16190634340750581No Hit
CTTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT1530.1587927598804384No Hit
TTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC1500.15567917635337097No Hit
TATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTCT1340.13907339754234474No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGA1310.13595981401527732No Hit
ATTATACACATCTCCGAGCCCACGAGACTATAATGCATCTCGTATGCCGT1180.1224676187313185No Hit
ATTATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCAGT1120.11624045167718366No Hit
TCTTAATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCC1080.1120890069744271No Hit
ATATACACATCTCCGAGCCCACGAGACTTTAATGCATCTCGTATGCCGTC1020.10586183992029226No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCTCGA150.002218705870.0000137
AACCAGC150.002218705870.0000116
TAAGTGC150.002218705870.0000118
CGCTCTT150.002218705870.0000112
TACCTTT150.002218705870.0000136
GTGTAGT150.002218705870.0000115
CGAATTC150.002218705870.0000148
AACTGTT150.002218705870.0000139
TCTGCTG150.002218705870.0000146
CGTCTAC207.8918674E-570.048
ATTATAC18900.067.037031
ATCTGCT700.065.0000152
ATCTTAT1850.064.3243261
AGGGGGG26100.063.2950270
TAAAGCA453.783498E-1062.22221831
AAGCATC453.783498E-1062.22221833
TTAAAGC401.0533768E-861.2530
CCGTCTA308.322208E-658.33333647
TACGAGC308.322208E-658.33333613
AATCTCG601.8189894E-1258.33333636
Produced by FastQC (version 0.11.5)