FastQCFastQC Report
Wed 25 May 2016
SRR1779885_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779885_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245310
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2589810.557254086665852No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG42191.7198646610411317No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC8890.3623985976927153No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG5210.21238433003138885No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT4110.1675431087195793No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCC150.002222813470.023
GCGCGTC252.3873182E-456.0000046
CGTTACA252.3873182E-456.0000042
GTCTGCC200.00694555252.50000423
AGAGCGT200.00694555252.50000425
CCCGAAC200.00694555252.5000049
CAGAGTC305.8703154E-446.66666444
GCCGTAT6450.041.78294854
TCGCCGT6500.041.46153652
ATCTCGG6750.040.96296342
GTCGCCG6750.040.96296351
CGGTGGT6700.040.7462746
GATCTCG6800.040.66176641
CCGTATC6650.040.52631455
CTCGGTG6850.040.36496444
TCGGTGG6850.040.36496445
TGGTCGC6800.040.1470649
ATGGTCC350.00125383940.08
CGTAAGA350.00125383940.037
CGCCGTA6750.039.92592653