FastQCFastQC Report
Wed 25 May 2016
SRR1779885_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779885_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences245310
Sequences flagged as poor quality0
Sequence length76
%GC48

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT115754.718519424401777No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG77113.1433696139578493No Hit
ATTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGT60212.454445395621866No Hit
TCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCG19950.8132566956096368No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC12080.492438139496963No Hit
ATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCCGTCTT7060.2877991113285231RNA PCR Primer, Index 34 (95% over 23bp)
ATCTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC5790.2360278830867066No Hit
TCTTTATACACATCTCCGAGCCCACGAGACCTCATGGGATCTCGTATGCC3450.1406383759324936No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGGGGG22300.059.9525470
AGCCGTC308.362702E-658.33078845
AAGCCGT308.362702E-658.33078844
ATTATAC8350.057.015121
TAGCTTC252.3854294E-456.00897228
AACTCGT252.3878341E-455.9975537
GGAACAT200.00694115352.508411
GGACCTA200.00694115352.508411
GTCCATG200.00694115352.5084130
CGCGGGA200.00694675352.49770763
GCCGACT407.4622585E-752.49770746
CGAATGC200.00694675352.49770741
AACGACT200.00694675352.49770736
GCCGTAT200.00694675352.49770746
CCGTCTT24950.051.9015447
AAGGGGG25800.051.8194469
GCCGTCT25100.051.7308146
CGTCTTC25050.051.69434748
TATGCCG26500.051.63925643
CTGCTTG24900.051.30298254