FastQCFastQC Report
Wed 25 May 2016
SRR1779884_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779884_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52107
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG590211.326693150632353No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG11692.243460571516303No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC1720.3300900071007734No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT1370.26292052891166257No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1310.2514057612221007No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGTGC150.002212969670.042
CAGATCT150.002212969670.01
GGAGTAA150.002212969670.01
GGCGGTG200.006915030552.541
CGCCGTA1900.044.21052653
TGGTCGC1900.044.21052649
CGGTGGT2000.043.74999646
GATCTCG2000.043.74999641
GTGGTCG2000.043.74999648
TCGGTGG2000.043.74999645
TCGCCGT1950.043.07692352
GCCGTAT1950.043.07692354
ATCTCGG2050.042.68292642
CGTATCA2050.042.68292656
CTCGGTG2050.042.68292644
GTCGCCG2000.042.051
AGATCTC2100.041.66666440
GGTGGTC2100.041.66666447
TCTCGGT2100.041.66666443
GGTCGCC2050.040.9756150