FastQCFastQC Report
Wed 25 May 2016
SRR1779884_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779884_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences52107
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT21564.13763985644923No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG19783.7960350816588937No Hit
CTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGT18433.5369528086437523No Hit
TCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCG3780.7254303644423974No Hit
ATCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1910.3665534381177193No Hit
ATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCCGTCTT1740.33392826299729406TruSeq Adapter, Index 10 (95% over 21bp)
CTCTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC1420.27251616865296413No Hit
TCTTTATACACATCTCCGAGCCCACGAGACACCGGCTAATCTCGTATGCC560.1074711651025774No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CAGCGTC150.002212969670.02
CAGCGCG150.002212969670.025
CGTATGA150.002212969670.041
TATGACG150.002212969670.043
GCAGCGT150.002212969670.01
CGTTTAC150.002212969670.014
AGGGGGG4750.064.1052670
ATTATAC3150.058.8888851
TATGCCG5500.055.36363643
CGTCTTC5500.054.72727248
GCCGTCT5450.054.58715446
TCGTATG5900.053.98304740
ATGCCGT5650.053.89380644
CCGTCTT5400.053.79629547
CTCGTAT5800.053.70689839
TCTTCTG5650.053.27433850
TGCCGTC5650.053.27433845
CGTATGC5800.053.10344741
ATCTCGT5750.052.95652437
GTATGCC5750.052.95652442