FastQCFastQC Report
Wed 25 May 2016
SRR1779883_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779883_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80128
Sequences flagged as poor quality0
Sequence length76
%GC66

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2712633.853334664536746No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG51456.420976437699681No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC7420.9260183706070287No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT5540.6913937699680511No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG5260.6564496805111821No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1740.21715255591054314No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1110.13852835463258786No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTCATG200.00692856752.53
TCAGCCC200.00692856752.54
CTTGCAA200.00692856752.512
CAATATA200.00692856752.548
TTCTGCC200.00692856752.548
GCACTTA200.00692856752.51
AAAAAGG5000.050.39999870
ATCTCGG8800.048.52272442
GATCTCG8800.048.52272441
CGTATCA8750.048.456
TCATTAA8700.048.27586460
CATTAAA8700.048.27586461
AGATCTC8850.048.2485940
CCGTATC8800.048.12555
GCCGTAT8800.048.12554
TCGCCGT8750.048.052
TCTCGGT8750.048.043
GTATCAT8900.047.97752857
CGCCGTA8800.047.72727253
GTAGATC8900.047.5842738
Produced by FastQC (version 0.11.5)