FastQCFastQC Report
Wed 25 May 2016
SRR1779883_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779883_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80128
Sequences flagged as poor quality0
Sequence length76
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
CTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT1136814.187300319488818No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG851010.620507188498403No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGT75119.373751996805112No Hit
TCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCG19442.4261182108626196No Hit
CTCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC11111.3865315495207668No Hit
ATCTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC7020.8760982428115016No Hit
ATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCTT5910.7375698881789138RNA PCR Primer, Index 14 (95% over 21bp)
TCTTTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCC3480.4343051118210863No Hit
CTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC1490.18595247603833867No Hit
ATTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGACGT1160.14476837060702874No Hit
TTATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTC950.11856030351437699No Hit
TATACACATCTCCGAGCCCACGAGACCGGTTCCCATCTCGTATGCCGTCT900.11232028753993611No Hit
CTTATACACATCTCCGAGCCCACGAGACCGGTTACCATCTCGTATGCCGT900.11232028753993611No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCCGTC150.00221733570.045
GACGTCA150.00221733570.046
AGAACGG150.00221733570.025
GACCACG150.00221733570.018
TCCAGAC150.00221733570.030
ACGTCAT150.00221733570.047
ATTATAC9500.067.7894741
AGGGGGG22300.062.46636270
AATCTCG352.9408875E-760.036
CATATCG308.309433E-658.33333236
ATATCGT308.309433E-658.33333237
GTTACCA553.45608E-1157.27272431
CCGGTTA553.45608E-1157.27272428
GGTTACC553.45608E-1157.27272430
ATCTTAT1300.056.538461
CTATACA252.3775862E-456.0000041
CGGTTAC509.622454E-1056.00000429
AACGAGA252.3775862E-456.00000421
GTTCCAA252.3775862E-456.00000431
CAACTCG252.3775862E-456.00000436
Produced by FastQC (version 0.11.5)