FastQCFastQC Report
Wed 25 May 2016
SRR1779882_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779882_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136621
Sequences flagged as poor quality0
Sequence length76
%GC55

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG2339017.12035485027924No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG49383.6143784630474087No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC8110.5936129877544448No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG5620.4113569656202194No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT5080.37183156322966454No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1450.1061330249376011No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GACTTCC150.002220698970.023
AGACGGT150.002220698970.06
GAGCGTC150.002220698970.024
GACGGTT150.002220698970.07
TGAGCGT150.002220698970.023
TTCGAAA150.002220698970.013
CAGACGG150.002220698970.05
CTGAGCG200.006938996352.522
TGTGACA200.006938996352.526
ACAGGGT200.006938996352.58
GTATGCC200.006938996352.531
CCGGTCT200.006938996352.59
TCAAGTA200.006938996352.527
TGTCGGT200.006938996352.543
CAAAGTC200.006938996352.522
ATCTCGG8300.048.49397742
CTCGGTG8300.048.49397744
TCTCGGT8350.048.20359443
TCGGTGG8650.048.15028845
CGGTGGT8600.048.0232646
Produced by FastQC (version 0.11.5)