FastQCFastQC Report
Wed 25 May 2016
SRR1779882_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779882_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences136621
Sequences flagged as poor quality0
Sequence length76
%GC49

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG88796.499000885661794No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT50553.700016834893611No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGT16011.1718549856903404No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT9500.6953543013153176No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGACGT5140.3762232746063929No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCG3820.27960562431836977No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATTCCGT2810.2056784828101097No Hit
CTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCGT2800.20494653091398832No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCGA2540.18591578161483227No Hit
ATCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCC2400.17566845506913287No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGACGT2330.17054479179628315No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTATGCCCT2110.15444185008161262No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCGCGTATGCCGT1790.13101938940572824No Hit
ATTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTCGTAAGCCGT1790.13101938940572824No Hit
TCTTATACACATCTCCGAGCCCACGAGACTTCGCGGCATCTAGTATGCCG1700.12443182234063578No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCTGCC150.002218027170.0212328
TACGCGG207.891799E-570.0212329
TTCGCGT150.002218027170.0212329
CTACTGG150.002221251469.9956059
TCCGACT150.002221251469.99560546
CTGAATG150.002221251469.99560554
GCCGACA301.0179792E-769.99560546
TCTGTGT150.002221251469.99560539
GTACTCA150.002221251469.99560547
CGAATTA207.9061305E-569.99560548
CCGACTT207.9061305E-569.99560547
TTGCCGT401.3460522E-1069.99560544
CGACTTA353.6834535E-969.99560548
CGTTTGC401.3460522E-1069.99560541
TGCCGAC353.6834535E-969.99560545
AGCCGTC600.064.16263645
TGACGTA600.064.16263645
GCCGTAT1050.063.3293646
ATTATAC14100.063.045691
ATGACGA401.058288E-861.24615544
Produced by FastQC (version 0.11.5)