FastQCFastQC Report
Wed 25 May 2016
SRR1779881_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779881_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66971
Sequences flagged as poor quality0
Sequence length76
%GC56

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1242018.54534052052381No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG28314.227202819130668No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC7691.1482582013110152No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT3340.49872332800764513No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG2840.42406414716817725No Hit
TCTTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG1610.2404025623030864No Hit
CTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG1140.17022293231398664No Hit
CTTTACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGG830.12393424019351659No Hit
TATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGT820.12244105657672724No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATTTATC150.00221573970.051
AACCGTC150.00221573970.033
GGAATGG150.00221573970.027
CCGTCTG150.00221573970.035
AAACCGT150.00221573970.032
CTGTTGG150.00221573970.047
TTTATCC150.00221573970.052
ATAACTG150.00221573970.05
ACCGTCT150.00221573970.034
GCATGAT150.00221573970.046
TAACTGA150.00221573970.06
TCAGGAA252.3747528E-456.00000421
ATCCTCG200.00692361952.534
AACCGTA200.00692361952.510
ATCTCAG200.00692361952.510
CGTATCG200.00692361952.513
TTGGACC200.00692361952.528
CAACCGT200.00692361952.59
CGTCTGA200.00692361952.536
CCTGTTG200.00692361952.546
Produced by FastQC (version 0.11.5)