FastQCFastQC Report
Wed 25 May 2016
SRR1779881_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779881_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66971
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT65239.740036732316973No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG55608.302100909348823No Hit
CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT7631.1392990996102792No Hit
TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG5700.8511146615699332No Hit
ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT5450.8137850711501993Illumina PCR Primer Index 11 (95% over 22bp)
ATCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC4150.619671200967583No Hit
ATTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT2170.32402084484329036No Hit
TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC1750.2613071329381374No Hit
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT1640.24488211315345448No Hit
ATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC1000.14931836167893564No Hit
TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT950.1418524435949889Illumina PCR Primer Index 11 (95% over 21bp)
ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGA780.11646832210956981No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCCTG150.00221573970.053
CAAGAAT150.00221573970.04
CGACTTC207.8742945E-570.048
ATTATAC9100.063.846151
TGCATGA401.0468284E-861.2555
CACGTAT401.0468284E-861.2539
AGGGGGG8800.059.2613670
CGTCTTA308.294572E-658.33333248
CATCACG553.45608E-1157.27272436
TATTAAC252.3747528E-456.0000042
CCGACTT252.3747528E-456.00000447
AGTGTTC252.3747528E-456.0000048
TCACGTA452.6524503E-854.44444338
GCCGTCT11700.053.84615346
CCGTCTT11600.053.706947
TGCCGTC12000.053.6666745
CGTCTTC11700.053.5470148
ATGCCGT12100.053.51239844
TATGCCG12250.053.4285743
TCTTCTG11550.053.33333250