##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1779881_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 66971 Sequences flagged as poor quality 0 Sequence length 76 %GC 50 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 25.470935180899197 27.0 22.0 32.0 13.0 32.0 2 29.045034417882366 32.0 27.0 32.0 22.0 32.0 3 29.764360693434472 32.0 32.0 32.0 27.0 32.0 4 30.626121754192113 32.0 32.0 32.0 27.0 32.0 5 30.260261904406384 32.0 32.0 32.0 27.0 32.0 6 33.95837004076391 37.0 37.0 37.0 27.0 37.0 7 33.60750175449075 37.0 32.0 37.0 22.0 37.0 8 33.02053127473086 37.0 32.0 37.0 22.0 37.0 9 34.19559212196324 37.0 37.0 37.0 27.0 37.0 10-11 33.78769915336488 37.0 34.5 37.0 24.5 37.0 12-13 33.691336548655386 37.0 34.5 37.0 24.5 37.0 14-15 33.907706320646255 37.0 37.0 37.0 27.0 37.0 16-17 33.3273207806364 37.0 34.5 37.0 22.0 37.0 18-19 33.66465335742336 37.0 37.0 37.0 22.0 37.0 20-21 33.811933523465385 37.0 34.5 37.0 24.5 37.0 22-23 33.46026638395723 37.0 34.5 37.0 22.0 37.0 24-25 33.43987696166998 37.0 32.0 37.0 22.0 37.0 26-27 33.67886099953711 37.0 34.5 37.0 24.5 37.0 28-29 33.67821146466381 37.0 37.0 37.0 22.0 37.0 30-31 33.312194830598315 37.0 34.5 37.0 22.0 37.0 32-33 33.4560481402398 37.0 34.5 37.0 22.0 37.0 34-35 33.32245300204566 37.0 32.0 37.0 22.0 37.0 36-37 32.59367487419928 37.0 32.0 37.0 17.5 37.0 38-39 32.160853205118634 37.0 32.0 37.0 13.0 37.0 40-41 32.59722118528916 37.0 32.0 37.0 17.5 37.0 42-43 33.008600737632705 37.0 32.0 37.0 22.0 37.0 44-45 33.06290035985725 37.0 32.0 37.0 22.0 37.0 46-47 32.43031312060444 37.0 32.0 37.0 13.0 37.0 48-49 31.987121291305193 37.0 32.0 37.0 13.0 37.0 50-51 31.842237684968122 37.0 29.5 37.0 13.0 37.0 52-53 31.55527019157546 37.0 27.0 37.0 13.0 37.0 54-55 31.532678323453435 37.0 27.0 37.0 13.0 37.0 56-57 31.06142210807663 37.0 27.0 37.0 13.0 37.0 58-59 31.203931552463004 37.0 27.0 37.0 13.0 37.0 60-61 31.088366606441596 37.0 27.0 37.0 13.0 37.0 62-63 31.57115019934001 37.0 27.0 37.0 13.0 37.0 64-65 31.507473384002033 37.0 27.0 37.0 13.0 37.0 66-67 31.273088351674605 37.0 27.0 37.0 13.0 37.0 68-69 30.790506338564455 37.0 27.0 37.0 13.0 37.0 70-71 28.954174194800736 34.5 20.0 37.0 13.0 37.0 72-73 29.27991220080333 37.0 24.5 37.0 13.0 37.0 74-75 29.993915276761584 37.0 27.0 37.0 13.0 37.0 76 29.573352645174776 37.0 22.0 37.0 13.0 37.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 11101 1 -2.2576814637393845 11101 2 0.3189404067182302 11101 3 -2.541285391270719 11101 4 -2.1728701691639465 11101 5 -0.0822105739632768 11101 6 -1.0239014198217475 11101 7 -2.5837180793571655 11101 8 -2.8829732785145303 11101 9 1.337460473390145 11101 10-11 -0.8987485657824408 11101 12-13 -0.20825447585729506 11101 14-15 -2.996412762500654 11101 16-17 0.6040555056404529 11101 18-19 -1.9846295940412801 11101 20-21 -1.1978584472599039 11101 22-23 0.9900901003782749 11101 24-25 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0.054382167972207185 21611 6 0.917630838242772 21611 7 2.2171891787073505 21611 8 -0.7599894075467901 21611 9 0.49723869919659336 21611 10-11 0.8812413535723991 21611 12-13 0.5457777822072174 21611 14-15 1.511147721370314 21611 16-17 1.363632118543677 21611 18-19 0.797628470474848 21611 20-21 0.30062945596590396 21611 22-23 0.8242634874750507 21611 24-25 0.1975938896700029 21611 26-27 -0.1769481127511554 21611 28-29 0.057156661960299004 21611 30-31 1.0965299915091293 21611 32-33 1.154814607346438 21611 34-35 0.9055981522444938 21611 36-37 -0.5219404663797746 21611 38-39 0.6772757255312882 21611 40-41 0.8756039289131792 21611 42-43 1.5114215778356908 21611 44-45 0.9996339946876844 21611 46-47 1.6340666226670564 21611 48-49 1.3520892241186786 21611 50-51 1.3129138470797237 21611 52-53 0.09147599951610985 21611 54-55 1.7533941633367363 21611 56-57 0.3008031746292268 21611 58-59 0.28252522305718486 21611 60-61 0.212466502033255 21611 62-63 2.0001468098799116 21611 64-65 0.24158300944809952 21611 66-67 -0.5416541910768871 21611 68-69 0.9392353581679345 21611 70-71 0.21244216669945004 21611 72-73 1.5877158649975236 21611 74-75 1.655138832581411 21611 76 1.0794135391651736 21612 1 -1.1700470551372355 21612 2 0.7807683637074767 21612 3 0.4474242861486353 21612 4 0.05669972330917261 21612 5 0.6554238346388743 21612 6 1.7051308382427735 21612 7 0.645314178707352 21612 8 0.9358439257865427 21612 9 1.6503636991965962 21612 10-11 0.5775955202390648 21612 12-13 0.2791111155405517 21612 14-15 0.6621893880369853 21612 16-17 0.7021737852103414 21612 18-19 0.1892951371415137 21612 20-21 -0.32957887736742464 21612 22-23 1.475305154141715 21612 24-25 1.9267605563366743 21612 26-27 1.969926887248846 21612 28-29 -0.07721833803969957 21612 30-31 1.422571658175798 21612 32-33 1.592314607346438 21612 34-35 1.5123689855778224 21612 36-37 1.4983720336202282 21612 38-39 0.02206739219795395 21612 40-41 0.31206226224651346 21612 42-43 0.8046507445023536 21612 44-45 1.2689048280210216 21612 46-47 0.7080249560003935 21612 48-49 0.6947975574520129 21612 50-51 1.8129138470797237 21612 52-53 0.5097051661827763 21612 54-55 0.5653733300034034 21612 56-57 -1.7512801587041054 21612 58-59 0.8788793897238527 21612 60-61 0.4369456686999236 21612 62-63 2.1454593098799144 21612 64-65 2.428041342781434 21612 66-67 1.4744916422564458 21612 68-69 1.4600686915012702 21612 70-71 1.7733796666994515 21612 72-73 0.6439658649975222 21612 74-75 1.2790971659147452 21612 76 1.2585802058318407 >>END_MODULE >>Per sequence quality scores pass #Quality Count 15 2.0 16 3.0 17 8.0 18 14.0 19 36.0 20 94.0 21 181.0 22 330.0 23 587.0 24 805.0 25 1156.0 26 1535.0 27 2103.0 28 2796.0 29 3694.0 30 5139.0 31 7353.0 32 9699.0 33 11964.0 34 10971.0 35 5651.0 36 2850.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.32704684256895 41.21011214143858 11.7472263285998 4.715614687392674 2 25.79253087249324 22.677656826292765 36.80508892173991 14.724723379474087 3 25.457667612363743 23.76885172465283 34.61549947737793 16.157981185605493 4 19.271027758283434 28.18234758328232 32.328918487106364 20.217706171327887 5 19.176957190425707 32.829134998730794 38.35988711531857 9.634020695524928 6 28.87647831800263 45.197706367220164 15.720941345120057 10.20487396965715 7 28.161443012647265 26.529393319496496 19.324782368487853 25.98438129936838 8 25.78877424556898 43.082826895223306 15.555986919711518 15.5724119394962 9 26.5846411133177 16.489226680204865 15.865075928386913 41.06105627809052 10-11 26.576428603425363 28.201012378492184 28.031536037986594 17.191022980095862 12-13 27.37976138925804 19.28446641083454 27.79038688387511 25.545385316032316 14-15 26.501769422585898 19.821265921070314 18.402741485120426 35.27422317122337 16-17 33.857191918890265 28.068865628406325 18.244464021740754 19.829478430962656 18-19 33.74147018858909 22.986068596855354 18.891012527810545 24.381448686745006 20-21 26.291977124426992 21.220378970001942 18.756626002299505 33.731017903271564 22-23 26.620676267909005 29.02173344980252 18.8232132058145 25.53437707647397 24-25 33.486885821157394 29.24988240915641 19.53351898998798 17.72971277969822 26-27 34.49622533023693 30.038306737654853 19.48640596181274 15.979061970295477 28-29 33.78085751407536 21.45577276325025 20.42009527934171 24.343274443332685 30-31 27.076971321481636 27.46149193999985 20.683476066391403 24.778060672127108 32-33 32.30919706721221 21.37321367240581 19.900100049278002 26.417489211103977 34-35 26.27543211259193 20.945981259566206 27.939672229066336 24.838914398775525 36-37 25.412491974138057 27.022143913036988 21.417479207418136 26.147884905406816 38-39 24.95109823655015 22.488091860656105 28.076331546490273 24.484478356303473 40-41 24.905181424518442 20.10153800209049 29.72972972972973 25.263550843661342 42-43 33.19670601234872 19.656415884605906 29.174039315818156 17.972838787227214 44-45 25.447581789132613 27.89864269609234 29.339564886294067 17.314210628480982 46-47 32.63974436505626 20.16783509157017 23.02431667674573 24.168103866627845 48-49 31.701034776246434 19.165011721491393 22.214092666975258 26.91986083528691 50-51 24.604679637455018 20.02359230114527 29.506054859566085 25.865673201833626 52-53 23.434023681892164 19.985516118917143 38.01272192441505 18.56773827477565 54-55 22.73969330008511 20.325961983545117 31.47407086649445 25.46027384987532 56-57 30.796165504472086 19.996714996043064 23.22124501649968 25.98587448298517 58-59 23.66248077526093 20.976990040465278 35.949888757820545 19.41064042645324 60-61 30.449746905376955 28.799032417016317 22.674739812754773 18.076480864851952 62-63 22.8815457436801 38.64657836974213 19.874273939466335 18.597601947111436 64-65 22.291158047199886 39.88472536415287 19.05391179698524 18.770204791662 66-67 22.362084798530695 40.08332026787914 18.821720011049642 18.73287492254052 68-69 23.65128189813501 39.24235863284108 18.3781039554434 18.728255513580507 70-71 30.556509533977394 32.29009571306984 18.02944558092309 19.123949172029683 72-73 41.763910975728116 20.831560164550066 17.686891989756685 19.717636869965133 74-75 43.00667453076705 19.800361350435264 17.515043824939152 19.677920293858538 76 43.5410413773537 19.662829070166794 17.588735086383252 19.207394466096254 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 0.0 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 1.0 12 2.0 13 5.0 14 7.5 15 7.5 16 8.5 17 9.0 18 15.0 19 22.5 20 36.0 21 48.0 22 67.5 23 110.5 24 181.5 25 229.0 26 245.0 27 277.0 28 364.5 29 436.0 30 485.5 31 591.5 32 829.0 33 1010.0 34 1121.0 35 1411.0 36 1776.5 37 1963.0 38 2142.0 39 2474.0 40 2715.0 41 2928.5 42 3054.0 43 3215.5 44 3397.5 45 3484.0 46 3550.0 47 3648.5 48 4571.5 49 7202.5 50 9009.0 51 6211.0 52 2695.5 53 2017.0 54 2056.0 55 1675.0 56 1122.5 57 835.0 58 719.0 59 639.0 60 478.0 61 368.0 62 339.0 63 321.0 64 271.0 65 216.0 66 185.0 67 177.0 68 171.5 69 148.5 70 137.5 71 144.0 72 115.5 73 78.0 74 63.0 75 57.0 76 49.5 77 44.5 78 41.0 79 35.0 80 31.5 81 22.0 82 14.5 83 13.0 84 13.5 85 9.5 86 5.0 87 5.0 88 3.5 89 2.5 90 1.5 91 0.5 92 1.0 93 1.0 94 2.5 95 6.0 96 8.0 97 12.0 98 50.5 99 2806.0 100 5527.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.002986367233578713 2 0.002986367233578713 3 0.0014931836167893565 4 0.0 5 0.0 6 0.00447955085036807 7 0.0 8 0.0 9 0.0 10-11 0.0 12-13 0.0 14-15 0.0 16-17 0.0 18-19 0.0 20-21 0.0 22-23 7.465918083946782E-4 24-25 0.002239775425184035 26-27 0.0171716115930776 28-29 0.014931836167893568 30-31 0.006719326275552104 32-33 0.005972734467157426 34-35 0.005226142658762748 36-37 0.0 38-39 0.0 40-41 0.0014931836167893565 42-43 7.465918083946782E-4 44-45 0.0 46-47 7.465918083946782E-4 48-49 0.0 50-51 0.0 52-53 0.0 54-55 0.0 56-57 0.0 58-59 0.0 60-61 0.0 62-63 0.0 64-65 7.465918083946782E-4 66-67 7.465918083946782E-4 68-69 0.0 70-71 0.0 72-73 7.465918083946782E-4 74-75 0.0 76 0.002986367233578713 >>END_MODULE >>Sequence Length Distribution pass #Length Count 76 66971.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 44.90152454047274 #Duplication Level Percentage of deduplicated Percentage of total 1 72.0694356689169 32.36027534305894 2 12.517042998237503 11.240686267190277 3 6.3682617804529285 8.578339878454853 4 3.5948255794619404 6.456525958997178 5 1.9021648764590469 4.27050514401756 6 1.2270958731003292 3.3059085275716353 7 0.6451398357221243 2.027743351599946 8 0.4489375145489009 1.6126383061325051 9 0.26271158258787536 1.0616535515372325 >10 0.9111768813807323 6.056352749697631 >50 0.023278241495128197 0.7331531558435741 >100 0.013301852282930399 1.4498812919024653 >500 0.0099763892121978 2.8041988323304117 >1k 0.0 0.0 >5k 0.0066509261414651995 18.042137641665796 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 6523 9.740036732316973 No Hit GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG 5560 8.302100909348823 No Hit CTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGT 763 1.1392990996102792 No Hit TCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCG 570 0.8511146615699332 No Hit ATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCTT 545 0.8137850711501993 Illumina PCR Primer Index 11 (95% over 22bp) ATCTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC 415 0.619671200967583 No Hit ATTATACACATCTCCGAGCCCACGAGACGCCGCTCCATCTCGTATGCCGT 217 0.32402084484329036 No Hit TCTTTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCC 175 0.2613071329381374 No Hit ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGACGT 164 0.24488211315345448 No Hit ATATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTC 100 0.14931836167893564 No Hit TATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGTCT 95 0.1418524435949889 Illumina PCR Primer Index 11 (95% over 21bp) ATTATACACATCTCCGAGCCCACGAGACGACGCTCCATCTCGTATGCCGA 78 0.11646832210956981 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0014931836167893565 0.0 2 0.0 0.0 0.0 0.0014931836167893565 0.0 3 0.0 0.0 0.0 0.0014931836167893565 0.0 4 0.0 0.0 0.0 0.002986367233578713 0.0 5 0.0 0.0 0.0 0.00447955085036807 0.0 6 0.0 0.0 0.0 0.00447955085036807 0.0 7 0.0 0.0 0.0 0.00447955085036807 0.0 8 0.0 0.0 0.0 0.00447955085036807 0.0 9 0.0 0.0 0.0 0.00447955085036807 0.0 10 0.0 0.0 0.0 0.00447955085036807 0.0 11 0.0 0.0 0.0 0.007465918083946783 0.0 12 0.0 0.0 0.0 0.007465918083946783 0.0 13 0.0 0.0 0.0 0.007465918083946783 0.0 14 0.0 0.0 0.0 0.00895910170073614 0.0 15 0.0 0.0 0.0 0.00895910170073614 0.0 16 0.0 0.0 0.0 0.01343865255110421 0.0 17 0.0 0.0 0.0 0.01343865255110421 0.0 18 0.0 0.0 0.0 0.014931836167893566 0.0 19 0.0 0.0 0.0 0.028370488718997774 0.0 20 0.0 0.0 0.0 0.03583640680294456 0.0 21 0.0 0.0 0.0 0.041809141270101984 0.0 22 0.0 0.0 0.0 0.04330232488689134 0.0 23 0.0 0.0 0.0 0.05674097743799555 0.0 24 0.0 0.0 0.0 0.0686864463723104 0.0 25 0.0 0.0 0.0 0.07615236445625718 0.0 26 0.0 0.0 0.0 0.08660464977378268 0.0 27 0.0 0.0 0.0 0.09556375147451882 0.0 28 0.0 0.0 0.0 0.12990697466067402 0.0 29 0.0 0.0 0.0 0.1582774633796718 0.0 30 0.0 0.0 0.0 0.1821684012483015 0.0 31 0.0 0.0 0.0 0.20755252273372057 0.0 32 0.0 0.0 0.0 0.2508548476206119 0.0 33 0.0 0.0 0.0 0.3195412939929223 0.0 34 0.0 0.0 0.0 0.406145943766705 0.0 35 0.0 0.0 0.0 0.5315733675770109 0.0 36 0.0 0.0 0.0 0.7465918083946783 0.0 37 0.0 0.0 0.0 1.1124217945080705 0.0 38 0.0 0.0 0.0 1.5140881874244076 0.0 39 0.0 0.0 0.0 1.9247136820414807 0.0 40 0.0 0.0 0.0 2.478684803870332 0.0 41 0.0 0.0 0.0 3.019217273148079 0.0 42 0.0 0.0 0.0 3.6075316181630854 0.0 43 0.0 0.0 0.0 4.2436278389153514 0.0 44 0.0 0.0 0.0 4.87375132520046 0.0 45 0.0 0.0 0.0 5.639754520613399 0.0 46 0.0 0.0 0.0 6.369921309223395 0.0 47 0.0 0.0 0.0 7.120992668468442 0.0 48 0.0 0.0 0.0 7.700347911782712 0.0 49 0.0 0.0 0.0 8.297621358498455 0.0 50 0.0 0.0 0.0 8.944169864568247 0.0 51 0.0 0.0 0.0 9.6803093876454 0.0 52 0.0 0.0 0.0 10.30296695584656 0.0 53 0.0 0.0 0.0 10.901733586179093 0.0 54 0.0 0.0 0.0 11.51244568544594 0.0 55 0.0 0.0 0.0 12.185871496617938 0.0 56 0.0 0.0 0.0 12.744322169297158 0.0 57 0.0 0.0 0.0 13.337116065162533 0.0 58 0.0 0.0 0.0 13.943348613579012 0.0 59 0.0 0.0 0.0 14.486867450090338 0.0 60 0.0 0.0 0.0 15.045318122769556 0.0 61 0.0 0.0 0.0 15.591823326514461 0.0 62 0.0 0.0 0.0 16.102492123456422 0.0 63 0.0 0.0 0.0 16.578817697212227 0.0 64 0.0 0.0 0.0 17.031252333099403 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GATCCTG 15 0.002215739 70.0 53 CAAGAAT 15 0.002215739 70.0 4 CGACTTC 20 7.8742945E-5 70.0 48 ATTATAC 910 0.0 63.84615 1 TGCATGA 40 1.0468284E-8 61.25 55 CACGTAT 40 1.0468284E-8 61.25 39 AGGGGGG 880 0.0 59.26136 70 CGTCTTA 30 8.294572E-6 58.333332 48 CATCACG 55 3.45608E-11 57.272724 36 TATTAAC 25 2.3747528E-4 56.000004 2 CCGACTT 25 2.3747528E-4 56.000004 47 AGTGTTC 25 2.3747528E-4 56.000004 8 TCACGTA 45 2.6524503E-8 54.444443 38 GCCGTCT 1170 0.0 53.846153 46 CCGTCTT 1160 0.0 53.7069 47 TGCCGTC 1200 0.0 53.66667 45 CGTCTTC 1170 0.0 53.54701 48 ATGCCGT 1210 0.0 53.512398 44 TATGCCG 1225 0.0 53.42857 43 TCTTCTG 1155 0.0 53.333332 50 >>END_MODULE