FastQCFastQC Report
Wed 25 May 2016
SRR1779880_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1779880_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences184379
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG1983110.755563269135857No Hit
CTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTG37162.015413902884819No Hit
ATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGTGGTC8610.46697291990953416No Hit
CTCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGG4480.24297777946512347No Hit
TCTTATACACATCTGACGCTGCCGACGAGCGATCTAGTGTAGATCTCGGT4070.22074097375514565No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CACGGCG150.002221934570.047
CGTCTAC150.002221934570.017
TCGTCTA150.002221934570.016
CCAAGCG150.002221934570.055
TCATCGT150.002221934570.013
GCGGCAT150.002221934570.024
GTGACGC150.002221934570.028
CGGCCAA200.00694282952.552
AGGACCC200.00694282952.538
CCGTGAC200.00694282952.526
ATCGTCT200.00694282952.515
TGCGATA200.00694282952.539
GAACGTA200.00694282952.530
GCGATAA200.00694282952.540
CGTGTCC200.00694282952.59
GCGTGTC200.00694282952.58
TAGCTAG200.00694282952.564
ACGGCGG305.8664975E-446.66666448
GTACGAT305.8664975E-446.66666420
CGCCGTA7350.041.9047653
Produced by FastQC (version 0.11.5)